Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198796
Type: Artigo de periódico
Title: Development Of A Disposable Amperometric Biosensor For Salicylate Based On A Plastic Electrochemical Microcell.
Author: Carvalhal, R F
Machado, D S
Mendes, R K
Almeida, A L J
Moreira, N H
Piazetta, M H O
Gobbi, A L
Kubota, L T
Abstract: The use of an amperometric biosensor for rapid salicylate determination in blood is described. Photolitography was used to make gold electrodes on a polyester film. The plastic microcell was characterized using cyclic voltammetry to demonstrate the electrochemical performance of the system. The biosensor was constructed by immobilizing salicylate hydroxylase onto the working electrode of the plastic electrochemical microcell. The optimized working conditions were 0.1 mol L(-1) phosphate buffer at pH 7.6 with 0.5 mmol L(-1) of NADH and 300 mV vs. Au as the applied potential. The resulting biosensor exhibited a high sensitivity (97.4 nA/mmol L(-1) salicylate) and an adequate linear response range (1.2x10(-4) to 1.0x10(-3)mol(-1)). The biosensor performance was verified by determining salicylate in spiked blood samples and the results were statistically equivalent to the values obtained from the standard Trinder spectrophotometric method, with a 95% confidence level. This study shows the potential development of a portable, inexpensive and disposable device for point-of-care monitoring.
Subject: Biosensing Techniques
Blood Chemical Analysis
Conductometry
Disposable Equipment
Equipment Design
Equipment Failure Analysis
Humans
Miniaturization
Polyesters
Reproducibility Of Results
Salicylates
Sensitivity And Specificity
Rights: fechado
Identifier DOI: 10.1016/j.bios.2010.02.026
Address: http://www.ncbi.nlm.nih.gov/pubmed/20363611
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_20363611.pdf270.96 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.