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Type: Artigo de periódico
Title: Transdentinal Protective Role Of Sodium Ascorbate Against The Cytopathic Effects Of H2o2 Released From Bleaching Agents.
Author: Lima, Adriano Fonseca
Lessa, Fernanda Campos Rosetti
Mancini, Maria Nadir Gasparoto
Hebling, Josimeri
Costa, Carlos Alberto de Souza
Marchi, Giselle Maria
Abstract: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.
Subject: Antioxidants
Ascorbic Acid
Cell Adhesion
Cell Culture Techniques
Cell Line
Cell Survival
Coloring Agents
Dentin Permeability
Hydrogen Peroxide
Materials Testing
Microscopy, Electron, Scanning
Protective Agents
Spectrophotometry, Ultraviolet
Tetrazolium Salts
Time Factors
Tooth Bleaching
Citation: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, And Endodontics. v. 109, n. 4, p. e70-6, 2010-Apr.
Rights: fechado
Identifier DOI: 10.1016/j.tripleo.2009.12.020
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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