Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198741
Type: Artigo de periódico
Title: Searching For Monooxygenases And Hydrolases In Bacteria From An Extreme Environment.
Author: da Cruz, Georgiana F
Angolini, Célio F F
de Oliveira, Luciana G
Lopes, Patrícia F
de Vasconcellos, Suzan P
Crespim, Elaine
de Oliveira, Valéria M
dos Santos Neto, Eugênio V
Marsaioli, Anita J
Abstract: Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
Subject: Bacteria
Bacterial Proteins
Dna, Bacterial
Hydrolases
Mixed Function Oxygenases
Molecular Sequence Data
Petroleum
Phylogeny
Rna, Ribosomal, 16s
Water Microbiology
Rights: fechado
Identifier DOI: 10.1007/s00253-010-2485-7
Address: http://www.ncbi.nlm.nih.gov/pubmed/20204614
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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