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Type: Artigo de periódico
Title: Searching For Monooxygenases And Hydrolases In Bacteria From An Extreme Environment.
Author: da Cruz, Georgiana F
Angolini, Célio F F
de Oliveira, Luciana G
Lopes, Patrícia F
de Vasconcellos, Suzan P
Crespim, Elaine
de Oliveira, Valéria M
dos Santos Neto, Eugênio V
Marsaioli, Anita J
Abstract: Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
Subject: Bacteria
Bacterial Proteins
Dna, Bacterial
Mixed Function Oxygenases
Molecular Sequence Data
Rna, Ribosomal, 16s
Water Microbiology
Citation: Applied Microbiology And Biotechnology. v. 87, n. 1, p. 319-29, 2010-Jun.
Rights: fechado
Identifier DOI: 10.1007/s00253-010-2485-7
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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