Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198740
Type: Artigo de periódico
Title: Molecular And Ultrastructural Profiles Of The Symbionts In Cephalotes Ants.
Author: Bution, M L
C Bresil,
Destéfano, R H R
Tango, M F de A
da Silveira, W D
Paulino, L C
Caetano, F H
Solferini, V N
Abstract: The molecular and ultrastructural profiles of the symbionts found in the midgut and ileum of Cephalotes atratus, Cephalotes clypeatus, and Cephalotes pusillus were determined using the V3 region of the bacterial 16S rDNA gene and transmission electron microscopy (T.E.M.). Two samples of C. atratus, three of C. clypeatus, and six of C. pusillus were analyzed. The coefficients of similarity ranged from 80% to 94% for the samples of symbionts from C. clypeatus and C. atratus, despite being collected in geographically distant sites. The variability within symbionts found in the samples of C. pusillus varied from 29% to 55%, in samples geographically close as well as distant. PCR-DGGE was effective for the purpose of this study and can be considered a versatile tool to analyze gut microbiota. Details of the ultrastructural aspect of these bacteria are presented.
Subject: Animals
Bacteria
Bacterial Physiological Phenomena
Biodiversity
Cluster Analysis
Dna, Bacterial
Dna, Ribosomal
Electrophoresis, Polyacrylamide Gel
Gastrointestinal Tract
Geography
Hymenoptera
Metagenome
Microscopy, Electron, Transmission
Nucleic Acid Denaturation
Phylogeny
Polymerase Chain Reaction
Rna, Ribosomal, 16s
Sequence Analysis, Dna
Symbiosis
Rights: fechado
Identifier DOI: 10.1016/j.micron.2010.01.011
Address: http://www.ncbi.nlm.nih.gov/pubmed/20202856
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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