Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198736
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNeurotoxic, Myotoxic And Cytolytic Activities Of The New Basic Pla(2) Isoforms Bmjetx-i And Bmjetx-ii Isolated From The Bothrops Marajoensis (marajó Lancehead) Snake Venom.pt_BR
dc.contributor.authorPonce-Soto, L Apt_BR
dc.contributor.authorMartins-de-Souza, Dpt_BR
dc.contributor.authorMarangoni, Spt_BR
unicamp.authorL A Ponce-Soto, Department of Biochemistry, Institute of Biology (IB), State University of Campinas (UNICAMP), P.O. Box 6109, Zip code 13083-970, Campinas, SP, Brazil. poncesoto@yahoo.com.arpt_BR
unicamp.author.externalD Martins-de-Souza,pt
unicamp.author.externalS Marangoni,pt
dc.subjectAmino Acid Sequencept_BR
dc.subjectAnimalspt_BR
dc.subjectBothropspt_BR
dc.subjectCell Linept_BR
dc.subjectChickenspt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectChromatography, Ion Exchangept_BR
dc.subjectCrotalid Venomspt_BR
dc.subjectEdemapt_BR
dc.subjectHumanspt_BR
dc.subjectInterleukin-6pt_BR
dc.subjectIsoenzymespt_BR
dc.subjectMalept_BR
dc.subjectMicept_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectMuscle, Skeletalpt_BR
dc.subjectPhospholipases A2, Secretorypt_BR
dc.subjectSpectrometry, Mass, Electrospray Ionizationpt_BR
dc.description.abstractThe BmjeTX-I and BmjeTX-II isoforms of PLA(2) were purified from Bothrops marajoensis venom by ion-exchange chromatography and reverse phase HPLC. Both isoforms showed a molecular mass of 13808.89 Da (BmjeTX-I) and 13863.97 Da (BmjeTX-II) determined by based on the determined primary structures and SDS-PAGE and confirmed experimentally by MALDI-TOF mass spectrometry. Multiple alignment of BmjeTX-I and BmjeTX-II isoforms of PLA(2) show high degree of homology with basic PLA(2) myotoxins from other Bothrops venoms. Ex vivo, both isoforms caused a blockade of the neuromuscular transmission in young chick biventer cervicis preparations in a similar way to other Bothrops species. In chick preparations, contractures to exogenous acetylcholine (55 and 110 microM) or KCl (13.4 mM) were unaltered after complete blockade for the both isoforms BmjeTX-I and BmjeTX-II of PLA(2). These results, which strongly suggested a presynaptic mechanism of action for these toxins. In mice, both isoforms induced myonecrosis and a systemic interleukin-6 response upon intramuscular injection. Both isoforms BmjeTX-I and BmjeTX-II of PLA(2) also induced moderate marked paw edema, evidencing the local increase in vascular permeability. Since both isoforms of PLA(2) exert a strong proinflammatory effect, the enzymatic hydrolysis of phospholipids might be relevant for this phenomenon and produced cytotoxicity in murine skeletal muscle C2C12 myoblasts and myotubes.en
dc.relation.ispartofThe Protein Journalpt_BR
dc.relation.ispartofabbreviationProtein J.pt_BR
dc.date.issued2010-Febpt_BR
dc.identifier.citationThe Protein Journal. v. 29, n. 2, p. 103-13, 2010-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume29pt_BR
dc.description.firstpage103-13pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1875-8355pt_BR
dc.identifier.doi10.1007/s10930-010-9229-5pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20195718pt_BR
dc.date.available2015-11-27T13:17:36Z-
dc.date.accessioned2015-11-27T13:17:36Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:17:36Z (GMT). No. of bitstreams: 1 pmed_20195718.pdf: 495703 bytes, checksum: 227952cd0614f78b61147e8304ab13fe (MD5) Previous issue date: 2010en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198736-
dc.identifier.idPubmed20195718pt_BR
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