Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/198736
Type: | Artigo de periódico |
Title: | Neurotoxic, Myotoxic And Cytolytic Activities Of The New Basic Pla(2) Isoforms Bmjetx-i And Bmjetx-ii Isolated From The Bothrops Marajoensis (marajó Lancehead) Snake Venom. |
Author: | Ponce-Soto, L A Martins-de-Souza, D Marangoni, S |
Abstract: | The BmjeTX-I and BmjeTX-II isoforms of PLA(2) were purified from Bothrops marajoensis venom by ion-exchange chromatography and reverse phase HPLC. Both isoforms showed a molecular mass of 13808.89 Da (BmjeTX-I) and 13863.97 Da (BmjeTX-II) determined by based on the determined primary structures and SDS-PAGE and confirmed experimentally by MALDI-TOF mass spectrometry. Multiple alignment of BmjeTX-I and BmjeTX-II isoforms of PLA(2) show high degree of homology with basic PLA(2) myotoxins from other Bothrops venoms. Ex vivo, both isoforms caused a blockade of the neuromuscular transmission in young chick biventer cervicis preparations in a similar way to other Bothrops species. In chick preparations, contractures to exogenous acetylcholine (55 and 110 microM) or KCl (13.4 mM) were unaltered after complete blockade for the both isoforms BmjeTX-I and BmjeTX-II of PLA(2). These results, which strongly suggested a presynaptic mechanism of action for these toxins. In mice, both isoforms induced myonecrosis and a systemic interleukin-6 response upon intramuscular injection. Both isoforms BmjeTX-I and BmjeTX-II of PLA(2) also induced moderate marked paw edema, evidencing the local increase in vascular permeability. Since both isoforms of PLA(2) exert a strong proinflammatory effect, the enzymatic hydrolysis of phospholipids might be relevant for this phenomenon and produced cytotoxicity in murine skeletal muscle C2C12 myoblasts and myotubes. |
Subject: | Amino Acid Sequence Animals Bothrops Cell Line Chickens Chromatography, High Pressure Liquid Chromatography, Ion Exchange Crotalid Venoms Edema Humans Interleukin-6 Isoenzymes Male Mice Molecular Sequence Data Muscle, Skeletal Phospholipases A2, Secretory Spectrometry, Mass, Electrospray Ionization |
Citation: | The Protein Journal. v. 29, n. 2, p. 103-13, 2010-Feb. |
Rights: | fechado |
Identifier DOI: | 10.1007/s10930-010-9229-5 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/20195718 |
Date Issue: | 2010 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
---|---|---|---|
pmed_20195718.pdf | 484.08 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.