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Type: Artigo de periódico
Title: A Procedure For Characterizing Glucans Synthesized By Purified Enzymes Of Cariogenic Streptococcus Mutans.
Author: Aires, C P
Koo, H
Sassaki, G L
Iacomini, M
Cury, J A
Abstract: Extracellular polysaccharide is a virulence factor of the most cariogenic bacteria, Streptococcus mutans. We describe a procedure for chemical analysis of this polysaccharide of S. mutans, using a well-known glucan synthesized by purified enzymes of cariogenic streptococci. Soluble and insoluble glucan from glucosyltransferase D and B respectively, were prepared and analyzed by nuclear magnetic resonance spectroscopy and methylation. Soluble alpha-glucan contained 60% of (1-->6)-linkages and 17% of branching while insoluble alpha-glucan was composed of 57% of (1-->3)- and 28% of (1-->6)-linkages with 8% branching. The described procedure could be important focusing future studies on in vivo biofilm.
Subject: Dental Caries
Magnetic Resonance Spectroscopy
Streptococcus Mutans
Rights: fechado
Identifier DOI: 10.1016/j.ijbiomac.2010.01.018
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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