Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198718
Type: Artigo
Title: A procedure for characterizing glucans synthesized by purified enzymes of cariogenic streptococcus mutans
Author: Aires, C. P.
Koo, H.
Sassaki, G. L.
Iacomini, M.
Cury, J. A.
Abstract: Extracellular polysaccharide is a virulence factor of the most cariogenic bacteria, Streptococcus mutans. We describe a procedure for chemical analysis of this polysaccharide of S. mutans, using a well-known glucan synthesized by purified enzymes of cariogenic streptococci. Soluble and insoluble glucan from glucosyltransferase D and B respectively, were prepared and analyzed by nuclear magnetic resonance spectroscopy and methylation. Soluble alpha-glucan contained 60% of (1-->6)-linkages and 17% of branching while insoluble alpha-glucan was composed of 57% of (1-->3)- and 28% of (1-->6)-linkages with 8% branching. The described procedure could be important focusing future studies on in vivo biofilm.
Extracellular polysaccharide is a virulence factor of the most cariogenic bacteria, Streptococcus mutans. We describe a procedure for chemical analysis of this polysaccharide of S. mutans, using a well-known glucan synthesized by purified enzymes of cariogenic streptococci. Soluble and insoluble glucan from glucosyltransferase D and B respectively, were prepared and analyzed by nuclear magnetic resonance spectroscopy and methylation. Soluble α-glucan contained 60% of (1 → 6)-linkages and 17% of branching while insoluble α-glucan was composed of 57% of (1 → 3)- and 28% of (1 → 6)-linkages with 8% branching. The described procedure could be important focusing future studies on in vivo biofilm
Subject: Streptococcus mutans
Beta-glucanas
Country: Holanda
Editor: Elsevier
Citation: International Journal Of Biological Macromolecules. v. 46, n. 5, p. 551-4, 2010-Jun.
Rights: fechado
Identifier DOI: 10.1016/j.ijbiomac.2010.01.018
Address: https://www.sciencedirect.com/science/article/pii/S0141813010000280?via%3Dihub
Date Issue: 2010
Appears in Collections:FOP - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_20144649.pdf383.32 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.