Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198716
Type: Artigo de periódico
Title: Purification Of Legumin-like Proteins From Coffea Arabica And Coffea Racemosa Seeds And Their Insecticidal Properties Toward Cowpea Weevil ( Callosobruchus Maculatus ) (coleoptera: Bruchidae).
Author: Coelho, Mirela Batista
Macedo, Maria Lígia Rodrigues
Marangoni, Sérgio
Silva, Desiree Soares da
Cesarino, Igor
Mazzafera, Paulo
Abstract: Legumin-like proteins from seeds of Coffea arabica (CaL-1 and CaL-2) and Coffea racemosa (CrL-1 and CrL-2) were characterized and isolated by gel filtration and reverse-phase chromatography. The insecticidal properties of the purified proteins were tested against Callosobruchus maculatus using artificial diets. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses indicated that CaL-1 is composed of two subunits of 33 and 24 kDa, while CaL-2, CrL-1, and CrL-2 were monomeric with a single band of 14 kDa. The LD(50) values were 0.5% (w/w) for CaL-1 and 0.3% (w/w) for CaL-2, CrL-1, and CrL-2. ED(50) at 0.3% was assessed for all protein concentrations. The legumin-like proteins were not digested by midgut homogenates of C. maculatus until 8 h of incubation. CaL-1 and CaL-2 ( C. arabica ) and CrL-1 and CrL-2 ( C. racemosa ) are chitin-binding proteins, and their insecticidal properties toward C. maculatus larvae might be related to their capacity to bind chitin present in the larval gut and their associated low digestibility.
Subject: Animals
Chromatography, Affinity
Chromatography, High Pressure Liquid
Coffee
Electrophoresis, Polyacrylamide Gel
Insecticides
Plant Proteins
Seeds
Weevils
Rights: fechado
Identifier DOI: 10.1021/jf9037216
Address: http://www.ncbi.nlm.nih.gov/pubmed/20141142
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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