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Type: Artigo de periódico
Title: Major Histocompatibility Complex Class I Expression And Glial Reaction Influence Spinal Motoneuron Synaptic Plasticity During The Course Of Experimental Autoimmune Encephalomyelitis.
Author: Freria, C M
Zanon, R G
Santos, L M B
Oliveira, A L R
Abstract: Recent studies have shown that major histocompatibility complex class I (MHC I) expression directly influences the stability of nerve terminals. Also, the acute phase of experimental autoimmune encephalomyelitis (EAE) has shown a significant impact on inputs within the spinal cord. Therefore, the present work investigated the synaptic covering of motoneurons during the induction phase of disease and progressive remissions of EAE. EAE was induced in C57BL/6J mice, which were divided into four groups: normal, peak disease, first remission, and second remission. The animals were killed and their lumbar spinal cords processed for in situ hybridization (IH), immunohistochemistry, and transmission electron microscopy (TEM). The results indicated an increase in glial reaction during the peak disease. During this period, the TEM analysis showed a reduction in the synaptic covering of the motoneurons, corresponding to a reduction in synaptophysin immunolabeling and an increase in the MHC I expression. The IH analysis reinforced the immunolabeling results, revealing an increased expression of MHC I mRNA by motoneurons and nonneuronal cells during the peak disease and first remission. The results observed in both remission groups indicated a return of the terminals to make contact with the motoneuron surface. The ratio between excitatory and inhibitory inputs increased, indicating the potential for development of an excitotoxic process. In conclusion, the results presented here indicate that MHC I up-regulation during the course of EAE correlates with the periods of synaptic plasticity induced by the infiltration of autoreactive immune cells and that synaptic plasticity decreases after recurrent peaks of inflammation.
Subject: Animals
Disease Models, Animal
Disease Progression
Encephalomyelitis, Autoimmune, Experimental
Glial Fibrillary Acidic Protein
Histocompatibility Antigens Class I
In Situ Hybridization
Mice, Inbred C57bl
Microscopy, Electron, Transmission
Motor Neurons
Neuronal Plasticity
Presynaptic Terminals
Rna, Messenger
Spinal Cord
Time Factors
Beta 2-microglobulin
Rights: fechado
Identifier DOI: 10.1002/cne.22259
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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