Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198701
Type: Artigo de periódico
Title: Image Analysis Of Two-dimensional Gel Electrophoresis For Comparative Proteomics Of Transgenic And Non-transgenic Soybean Seeds.
Author: Brandão, A R
Barbosa, H S
Arruda, M A Z
Abstract: Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150-500 microg), the pH separation range (3-10 or 4-7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry.
Subject: Electrophoresis, Gel, Two-dimensional
Hydrogen-ion Concentration
Image Processing, Computer-assisted
Plants, Genetically Modified
Proteomics
Seeds
Soybean Proteins
Soybeans
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Rights: fechado
Identifier DOI: 10.1016/j.jprot.2010.01.009
Address: http://www.ncbi.nlm.nih.gov/pubmed/20123049
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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