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Type: Artigo de periódico
Title: Image Analysis Of Two-dimensional Gel Electrophoresis For Comparative Proteomics Of Transgenic And Non-transgenic Soybean Seeds.
Author: Brandão, A R
Barbosa, H S
Arruda, M A Z
Abstract: Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150-500 microg), the pH separation range (3-10 or 4-7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry.
Subject: Electrophoresis, Gel, Two-dimensional
Hydrogen-ion Concentration
Image Processing, Computer-assisted
Plants, Genetically Modified
Soybean Proteins
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Citation: Journal Of Proteomics. v. 73, n. 8, p. 1433-40, 2010-Jun.
Rights: fechado
Identifier DOI: 10.1016/j.jprot.2010.01.009
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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