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Type: Artigo de periódico
Title: Fast Determination Of Ethambutol In Pharmaceutical Formulations Using Capillary Electrophoresis With Capacitively Coupled Contactless Conductivity Detection.
Author: da Silva, José Alberto Fracassi
de Castro, Nathan Vitorazzi
de Jesus, Dosil Pereira
Faria, Adriana F
De Souza, Marcus V N
de Oliveira, Marcone Augusto Leal
Abstract: A method for the determination of ethambutol (EMB), a first-line drug against tuberculosis, based on CE with capacitively coupled contactless conductivity detection is proposed. The separation of EMB and its main product of degradation were achieved in less than 3 min with a resolution of 2.0 using a BGE composed of 50 mmol/L histidine and 30 mmol/L MES, pH 6.30. By raising the pH to 8.03, the analysis time was reduced to 1.0 min, but with a significant loss of resolution (0.7). Using the best separation conditions, linearity of 0.9976 (R(2), five data points), sensitivity of 1.26x10(-4) V min mumol(-1) L, and LOD and quantification of 23.5 and 78.3 mumol/L, respectively, were obtained. Recoveries at four levels of concentration ranged from 95 to 102% and the concentration range studied ranged from 100 to 500 mumol/L. The results obtained for the determination of EMB in pharmaceutical formulations were compared with those obtained by using CE with photometric detection.
Subject: Antitubercular Agents
Electric Conductivity
Electrophoresis, Capillary
Hydrogen-ion Concentration
Pharmaceutical Preparations
Sensitivity And Specificity
Time Factors
Citation: Electrophoresis. v. 31, n. 3, p. 570-4, 2010-Jan.
Rights: fechado
Identifier DOI: 10.1002/elps.200900404
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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