Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198681
Type: Artigo de periódico
Title: Transgenic Soybean Seed As Protein Expression System: Aqueous Extraction Of Recombinant Beta-glucuronidase.
Author: Robić, Goran
Farinas, Cristiane S
Rech, Elíbio L
Miranda, Everson A
Abstract: Soybean is one of the plant species with potential to be used as seed-based bioreactor. As part of the downstream processing (DSP) of this technology, extraction is a key step, since it defines the composition of the solution from which the recombinant product will be purified. In the present work, the characteristics of soybean seeds used as a bioreactor were evaluated from a process engineering standpoint through analysis of the influence of pH and ionic strength on the extraction of recombinant beta-glucuronidase (rGUS). Concentrations of recombinant protein and native soybean compounds were analyzed and compared with similar data from extraction studies using transgenic corn seeds as bioreactor. Efficient rGUS extraction was obtained at pH of around 5.5 with no addition of salt. Soybean seed extracts had lower levels of co-extracted native compounds, than corn seed extracts, and should be considered as a potential plant bioreactor in terms of DSP.
Subject: Bioreactors
Gene Expression Regulation, Plant
Glucuronidase
Hydrogen-ion Concentration
Plant Proteins
Plants, Genetically Modified
Recombinant Proteins
Seeds
Soybeans
Zea Mays
Rights: fechado
Identifier DOI: 10.1007/s12010-009-8637-5
Address: http://www.ncbi.nlm.nih.gov/pubmed/19412577
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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