Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198668
Type: Artigo de periódico
Title: Cholesteryl Ester Transfer Protein (cetp) Increases Postprandial Triglyceridaemia And Delays Triacylglycerol Plasma Clearance In Transgenic Mice.
Author: Salerno, Alessandro G
Patrício, Patrícia R
Berti, Jairo A
Oliveira, Helena C F
Abstract: The CETP (cholesteryl ester transfer protein) is a plasma protein synthesized in several tissues, mainly in the liver; CETP reduces plasma HDL (high-density lipoprotein) cholesterol and increases the risk of atherosclerosis. The effect of CETP levels on postprandial intravascular metabolism of TAGs (triacylglycerols) is an often-overlooked aspect of the relationship between CETP and lipoprotein metabolism. Here, we tested the hypothesis that CETP delays the plasma clearance of TAG-rich lipoprotein by comparing human CETP expressing Tg (transgenic) and non-Tg mice. After an oral fat load, the postprandial triglyceridaemia curve was markedly increased in CETP-Tg compared with non-Tg mice (280+/-30 versus 190+/-20 mg/dl per 6 h respectively, P<0.02). No differences in intestinal fat absorption and VLDL (very-low-density lipoprotein) secretion rates were observed. Kinetic studies of double-labelled chylomicron-like EMs (emulsions) showed that both [(3)H]triolein and [(14)C]cholesteryl oleate FCRs (fractional clearance rates) were significantly reduced ( approximately 20%) in CETP-Tg mice. Furthermore, TAG from lipid EM pre-incubated with CETP-Tg plasma had plasma clearance and liver uptake significantly lower than the non-Tg plasma-treated lipid EM. In addition, reductions in post-heparin plasma LPL (lipoprotein lipase) activity (50%) and adipose tissue mRNA abundance (39%) were verified in CETP-Tg mice. Therefore we conclude that CETP expression in Tg mice delays plasma clearance and liver uptake of TAG-rich lipoproteins by two mechanisms: (i) transferring TAG to HDLs and increasing CE content of the remnant particles and (ii) by diminishing LPL expression. These findings show that the level of CETP expression can influence the responsiveness to dietary fat and may lead to fat intolerance.
Subject: Animals
Blood Glucose
Cholesterol Ester Transfer Proteins
Cholesterol, Vldl
Chylomicrons
Dietary Fats
Emulsions
Fasting
Female
Hyperlipidemias
Intestinal Absorption
Ldl-receptor Related Proteins
Lipoprotein Lipase
Liver
Mice
Mice, Transgenic
Organ Size
Postprandial Period
Triglycerides
Rights: fechado
Identifier DOI: 10.1042/BJ20081299
Address: http://www.ncbi.nlm.nih.gov/pubmed/19191759
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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