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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleSynthesis And Application Of A Peroxidase-like Molecularly Imprinted Polymer Based On Hemin For Selective Determination Of Serotonin In Blood Serum.pt_BR
dc.contributor.authorSantos, Wilney de Jesus Rodriguespt_BR
dc.contributor.authorLima, Phabyanno Rodriguespt_BR
dc.contributor.authorTarley, César Ricardo Teixeirapt_BR
dc.contributor.authorHöehr, Nelci Fenaltipt_BR
dc.contributor.authorKubota, Lauro Tatsuopt_BR
unicamp.authorWilney de Jesus Rodrigues Santos, Departamento de Química Analítica, Instituto de Química, Universidade Estadual de Campinas, Campinas, SP 13084-971, Brazil.pt_BR
unicamp.author.externalPhabyanno Rodrigues Lima,pt
unicamp.author.externalCésar Ricardo Teixeira Tarley,pt
unicamp.author.externalNelci Fenalti Höehr,pt
unicamp.author.externalLauro Tatsuo Kubota,pt
dc.subjectBiocatalysispt_BR
dc.subjectBiomimetic Materialspt_BR
dc.subjectBlood Chemical Analysispt_BR
dc.subjectFlow Injection Analysispt_BR
dc.subjectHeminpt_BR
dc.subjectHumanspt_BR
dc.subjectMolecular Imprintingpt_BR
dc.subjectMultivariate Analysispt_BR
dc.subjectPeroxidasept_BR
dc.subjectPolymerspt_BR
dc.subjectPorositypt_BR
dc.subjectSerotoninpt_BR
dc.subjectSubstrate Specificitypt_BR
dc.subjectSurface Propertiespt_BR
dc.description.abstractThis work reports the preparation of a molecularly imprinted polymer (MIP) for selective catalytic detection of serotonin (5-hydroxytryptamine, 5-HT). The process is based on the synthesis of polymers with hemin introduced as the catalytic center to mimic the active site of peroxidase. The copolymer MIP, containing artificial recognition sites for 5-HT, has been prepared by bulk polymerization using methacrylic acid (MAA) and hemin as the functional monomers, and ethylene glycol dimethacrylate (EGDMA) as the cross-linker. For the determination of 5-HT, a flow injection analysis system coupled to an amperometric detector was optimized using multivariate analysis. The effects of different parameters, such as pH, buffer flow rate, buffer nature, peroxide concentration and sample volume were evaluated. After optimizing the experimental conditions, a linear response range from 1.0 up to 1000.0 micromolL(-1) was obtained with a sensitivity of 0.4nA/ micromolL(-1). The detection limit was found to be 0.30 micromolL(-1), while the precision values (n=6) evaluated by relative standard deviation (R.S.D.) were, respectively, 1.3 and 1.7% for solutions of 50 and 750 micromolL(-1) of 5-HT. No interference was observed by structurally similar compounds (including epinephrine, dopamine and norepinephrine), thus validating the good performance of the imprinted polymer. The method was applied for the determination of 5-HT in spiked blood serum samples.en
dc.relation.ispartofAnalytica Chimica Actapt_BR
dc.relation.ispartofabbreviationAnal. Chim. Actapt_BR
dc.date.issued2009-Janpt_BR
dc.identifier.citationAnalytica Chimica Acta. v. 631, n. 2, p. 170-6, 2009-Jan.pt_BR
dc.language.isoengpt_BR
dc.description.volume631pt_BR
dc.description.firstpage170-6pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1873-4324pt_BR
dc.identifier.doi10.1016/j.aca.2008.10.034pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19084622pt_BR
dc.date.available2015-11-27T13:15:53Z-
dc.date.accessioned2015-11-27T13:15:53Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:15:53Z (GMT). No. of bitstreams: 1 pmed_19084622.pdf: 711238 bytes, checksum: dce877358dad42bd87ca4ce5e8cc29b6 (MD5) Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198629-
dc.identifier.idPubmed19084622pt_BR
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