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dc.typeArtigo de periódicopt_BR
dc.titleNanocytotoxicity: Violacein And Violacein-loaded Poly (d, L-lactide-co-glycolide) Nanoparticles Acting On Human Leukemic Cells.pt_BR
dc.contributor.authorMelo, Patricia Spt_BR
dc.contributor.authorDe Azevedo, Marcelo M Mpt_BR
dc.contributor.authorFrungillo, Lucaspt_BR
dc.contributor.authorAnazetti, Maristela Cpt_BR
dc.contributor.authorMarcato, Priscyla Dpt_BR
dc.contributor.authorDuran, Nelsonpt_BR
unicamp.authorPatricia S Melo, Biochemistry Department, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, Campinas, SP, 13083-970, Brazil.pt_BR M M De Azevedo,pt Frungillo,pt C Anazetti,pt D Marcato,pt Duran,pt
dc.subjectCell Survivalpt_BR
dc.subjectDrug Carrierspt_BR
dc.subjectHl-60 Cellspt_BR
dc.subjectLactic Acidpt_BR
dc.subjectMacromolecular Substancespt_BR
dc.subjectMaterials Testingpt_BR
dc.subjectMolecular Conformationpt_BR
dc.subjectParticle Sizept_BR
dc.subjectPolyglycolic Acidpt_BR
dc.subjectSurface Propertiespt_BR
dc.description.abstractViolacein is a compound obtained from Chromobacterium violaceum, a bacterium found in the Amazonian region. Violacein-loaded poly (D, L-lactide-co-glycolide) nanoparticles has a similar inhibitory effect evaluated by trypan blue assay on leukemic HL60 cells than the free form. However, the cytotoxic effects evaluated by phosphatase activity and MTT reduction assays were lower for the encapsulated form than for free violacein. Based on morphological changes, violacein and violacein entrapped in nanoparticles were found to induce terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells. Thus, both formulations inhibit HL60 cell growth in vitro, partly by inducing cytotoxic effects and cell differentiation. Flow cytometric analysis of HL60 cells after treatment for 12 h showed that violacein-loaded PLGA induced apoptosis, with maximum cell death at a concentration of 2 microM. Violacein and violacein/PLGA induced opposite effects on the mitochondrial swelling which indicates altered mitochondrial function. The mitochondrial activity was also checked by flow cytometry studies. Labelled cells with the probe JC1 displayed a basal hypopolarized status of the mitochondria in treated cells. Based on morphological changes, alterations in phospholipid asymmetry and changes in mitochondrial polarization, violacein and nanoparticles containing violacein were found to trigger cell death by apoptosis. These methodologies are promising as diagnostic and mechanistic effects of nanoparticles in cell cultures.en
dc.relation.ispartofJournal Of Biomedical Nanotechnologypt_BR
dc.relation.ispartofabbreviationJ Biomed Nanotechnolpt_BR
dc.identifier.citationJournal Of Biomedical Nanotechnology. v. 5, n. 2, p. 192-201, 2009-Apr.pt_BR
dc.description.provenanceMade available in DSpace on 2015-11-27T13:15:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
Appears in Collections:Unicamp - Artigos e Outros Documentos

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