Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198443
Type: Artigo de periódico
Title: Characterization Of Mitochondrial Control Region, Two Intergenic Spacers And Trnas Of Zaprionus Indianus (diptera: Drosophilidae).
Author: da Silva, Norma Machado
de Souza Dias, Aline
da Silva Valente, Vera Lúcia
Valiati, Victor Hugo
Abstract: The control region in insects is the major noncoding region in animal mitochondrial DNA (mtDNA), and is responsible for a large part of the variation in the DNA sequence and size of the genome of this organelle. In this study, the mtDNA control region, two intergenic spacers and tRNA genes of a Zaprionus indianus strain were cloned, sequenced and compared with other Drosophila species. The overall A+T content in the Z. indianus control region is 94.3%, and a comparison with other Drosophila species demonstrated that the most conserved region appears to be the 420 base pairs nearest to the tRNA(ile), similar to the findings of other authors. We also describe conserved sequence blocks, including a poly-T involved in the replication process of Drosophila mtDNA; a putative secondary structure also involved in the replication process and repeated sequences. tRNA(ile) sequence demonstrated the greatest variability when the tRNA sequences of species were compared.
Subject: Animals
Base Composition
Base Sequence
Conserved Sequence
Dna, Intergenic
Drosophilidae
Female
Locus Control Region
Mitochondria
Models, Biological
Molecular Sequence Data
Nucleic Acid Conformation
Rna, Transfer
Sequence Homology, Nucleic Acid
Rights: fechado
Identifier DOI: 10.1007/s10709-009-9396-5
Address: http://www.ncbi.nlm.nih.gov/pubmed/19690968
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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