Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198369
Type: Artigo de periódico
Title: Ascorbate-induced Osteoblast Differentiation Recruits Distinct Mmp-inhibitors: Reck And Timp-2.
Author: Zambuzzi, Willian F
Yano, Claudia L
Cavagis, Alexandre D M
Peppelenbosch, Maikel P
Granjeiro, José Mauro
Ferreira, Carmen V
Abstract: The bone formation executed by osteoblasts represents an interesting research field both for basic and applied investigations. The goal of this work was to evaluate the molecular mechanisms involved during osteoblast differentiation in vitro. Accordingly, we demonstrated that, during the osteoblastic differentiation, TIMP-2 and RECK presented differential expressions, where RECK expression was downregulated from the 14th day in contrast with an increase in TIMP-2. Concomitantly, our results showed a temporal regulation of two major signaling cascades during osteoblast differentiation: proliferation cascades in which RECK, PI3 K, and GSK-3beta play a pivotal role and latter, differentiation cascades with participation of Ras, Rho, Rac-1, PKC alpha/beta, and TIMP-2. Furthermore, we observed that phosphorylation level of paxillin was downregulated while FAK(125) remained unchangeable, but active during extracellular matrix (ECM) remodeling. Concluding, our results provide evidences that RECK and TIMP-2 are involved in the control of ECM remodeling in distinct phases of osteoblast differentiation by modulating MMP activities and a multitude of signaling proteins governs these events.
Subject: 3t3 Cells
Animals
Ascorbic Acid
Cell Cycle
Cell Differentiation
Cells, Cultured
Extracellular Matrix
Gpi-linked Proteins
Membrane Glycoproteins
Mice
Osteoblasts
Protein Kinase C
Protein Tyrosine Phosphatase, Non-receptor Type 11
Signal Transduction
Tissue Inhibitor Of Metalloproteinase-2
Rac1 Gtp-binding Protein
Rho Gtp-binding Proteins
Rights: fechado
Identifier DOI: 10.1007/s11010-008-9951-x
Address: http://www.ncbi.nlm.nih.gov/pubmed/18989628
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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