Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198358
Type: Artigo de periódico
Title: Expression Of Basal Lamina Components By Schwann Cells Cultured On Poly(lactic Acid) (plla) And Poly(caprolactone) (pcl) Membranes.
Author: Pierucci, A
Duek, E A R
de Oliveira, A L R
Abstract: The present in vitro study investigated the expression of basal lamina components by Schwann cells (SCs) cultivated on PCL and PLLA membranes prepared by solvent evaporation. Cultures of SCs were obtained from sciatic nerves from neonatal Sprague Dawley rats and seeded on 24 well culture plates containing the polymer membranes. The purity of the cultures was evaluated with a Schwann cell marker antibody (anti-S-100). After one week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, laminin I and II. Positive labeling against the studied molecules was observed, indicating that such biomaterials positively stimulate Schwann cell adhesion and proliferation. Overall, the present results provide evidence that membrane-derived biodegradable polymers, particularly those derived from PLLA, are able to provide adequate substrate and stimulate SCs to produce ECM molecules, what may have in turn positive effects in vivo, influencing the peripheral nerve regeneration process.
Subject: Animals
Animals, Newborn
Basement Membrane
Biocompatible Materials
Cell Culture Techniques
Cells, Cultured
Extracellular Matrix Proteins
Gene Expression
Lactic Acid
Materials Testing
Polyesters
Polymers
Rats
Rats, Sprague-dawley
Schwann Cells
Citation: Journal Of Materials Science. Materials In Medicine. v. 20, n. 2, p. 489-95, 2009-Feb.
Rights: fechado
Identifier DOI: 10.1007/s10856-008-3614-z
Address: http://www.ncbi.nlm.nih.gov/pubmed/18987957
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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