Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198331
Type: Artigo de periódico
Title: Reactive Oxygen Species Generation In Peripheral Blood Monocytes And Oxidized Ldl Are Increased In Hyperlipidemic Patients.
Author: Vasconcelos, Edilma M A
Degasperi, Giovanna R
de Oliveira, Helena C F
Vercesi, Aníbal E
de Faria, Eliana C
Castilho, Lucia N
Abstract: Experimental and in vitro evidences have established that reactive oxygen species (ROS) generated by vascular wall cells play a key role in atherogenesis. Here, we evaluated the rate of ROS generation by resting peripheral monocytes in naive hyperlipidemic subjects. Primary hypercholesterolemic, combined hyperlipidemic, and normolipidemic individuals were studied. ROS generation and the mitochondrial electrical transmembrane potential were estimated by flow cytometry. Plasma oxidized (ox) LDL levels and lipid profile were measured by ELISA and enzymatic colorimetric methods. Both hyperlipidemic groups presented significantly higher rates of monocyte ROS generation and elevated plasma levels of ox-LDL. Combined hyperlipidemic subjects presented increased levels of small dense LDL and insulin. Significant positive correlations between monocyte ROS generation and ox-LDL concentrations were found in pooled data. These data provide evidence that ROS production by circulating monocytes from hyperlipidemic subjects may contribute to the systemic oxidative stress and possibly to atherogenesis.
Subject: Adult
Female
Humans
Hyperlipidemias
Lipoproteins
Lipoproteins, Ldl
Male
Middle Aged
Monocytes
Reactive Oxygen Species
Rights: fechado
Identifier DOI: 10.1016/j.clinbiochem.2009.05.010
Address: http://www.ncbi.nlm.nih.gov/pubmed/19467225
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_19467225.pdf290.98 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.