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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFluorometric Quantification Of Green Fluorescent Protein In Tobacco Leaf Extracts.pt_BR
dc.contributor.authorRobić, Goranpt_BR
dc.contributor.authorLacorte, Cristianopt_BR
dc.contributor.authorMiranda, Everson Apt_BR
unicamp.authorGoran Robić, Departamento de Processos Biotecnológicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas, CEP 13083-970 Campinas, SP, Brazil. goran@feq.unicamp.brpt_BR
unicamp.author.externalCristiano Lacorte,pt
unicamp.author.externalEverson A Miranda,pt
dc.subjectFluorometrypt_BR
dc.subjectGreen Fluorescent Proteinspt_BR
dc.subjectPlant Extractspt_BR
dc.subjectPlant Leavespt_BR
dc.subjectTobaccopt_BR
dc.description.abstractThe main use of green fluorescent protein (GFP) is as a reporter system, where the existence of the protein is usually determined visually using fluorescent microscopy. Although fluorescence-based quantification of GFP is possible, background fluorescence in plants and in plant extracts was observed by our group. Another phenomenon we observed that makes quantification difficult is the increased level of GFP fluorescence in Nicotiana benthamiana leaf extracts, probably the result of dimerization of GFP molecules promoted by interaction with some component(s) of tobacco extracts. In the current work, the background fluorescence was minimized and the enhancement of GFP fluorescence in tobacco extracts was eliminated with the addition of urea to the measured solution so that a simple quantification assay for the GFP in the tobacco extracts could be established.en
dc.relation.ispartofAnalytical Biochemistrypt_BR
dc.relation.ispartofabbreviationAnal. Biochem.pt_BR
dc.date.issued2009-Seppt_BR
dc.identifier.citationAnalytical Biochemistry. v. 392, n. 1, p. 8-11, 2009-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume392pt_BR
dc.description.firstpage8-11pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1096-0309pt_BR
dc.identifier.doi10.1016/j.ab.2009.05.016pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19457429pt_BR
dc.date.available2015-11-27T13:15:13Z-
dc.date.accessioned2015-11-27T13:15:13Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:15:13Z (GMT). No. of bitstreams: 1 pmed_19457429.pdf: 249048 bytes, checksum: 62bd7d47512d248842962793a26487de (MD5) Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198316-
dc.identifier.idPubmed19457429pt_BR
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