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Type: Artigo de periódico
Title: Fak Signalling Mediates Nf-kappab Activation By Mechanical Stress In Cardiac Myocytes.
Author: Crosara-Alberto, Daniella Pereira
Inoue, Rosana Yuri
Costa, Cláudia Raquel Cantarelli
Abstract: Nuclear Factor (NF)-kappaB and Focal Adhesion Kinase (FAK) signalling are implicated in cardiomyocyte hypertrophy. We investigated whether FAK signalling contributed towards NF-kappaB activation by mechanical stress in cardiac myocytes. Experiments were performed with pressure overload rat left ventricle and isolated cardiac myocytes from adult rats and isolated neonatal rat ventricular myocytes (NRVMs) underwent in vitro stretching. Pressure overload induced NF-kappaB increase in myocardial cell nuclear extracts. Adult rat isolated cardiac myocyte confocal microscopy analysis showed NF-kappaB detected into nuclei. Cyclic stretch increased the amount and NF-kappaB DNA-binding activity in NRVMs nuclear extracts. NRVMs confocal microscopy confirmed NF-kappaB subcellular relocation in stretched cells. Changes in NF-kappaB subcellular location and DNA-binding activity in stretched NRVMs were paralleled by increased FAK phosphorylation, detected in stretched NRVMs by anti-phosphospecific antibody directed to Tyr397. NRVMs treatment with FAK/Src pharmacological inhibitor attenuated NF-kappaB subcelullar relocation and increased DNA binding activity induced by cyclic stretch in cardiac myocytes. FAK signalling coordinates cardiac myocyte NF-kappaB activation in response to mechanical stress. Further studies are needed to elucidate the influence of this signalling pathway on gene transcription regulation, and cardiac myocyte phenotypic changes in response to mechanical stress.
Subject: Animals
Blood Pressure
Cell Nucleus
Constriction, Pathologic
Focal Adhesion Protein-tyrosine Kinases
Heart Ventricles
Myocytes, Cardiac
Nf-kappa B
Nf-kappa B P50 Subunit
Rats, Wistar
Signal Transduction
Stress, Mechanical
Transcription Factor Rela
Citation: Clinica Chimica Acta; International Journal Of Clinical Chemistry. v. 403, n. 1-2, p. 81-6, 2009-May.
Rights: fechado
Identifier DOI: 10.1016/j.cca.2009.01.023
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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