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Type: Artigo de periódico
Title: Cytotoxic Effects Of Different Concentrations Of A Carbamide Peroxide Bleaching Gel On Odontoblast-like Cells Mdpc-23.
Author: de Lima, Adriano Fonseca
Lessa, Fernanda Campos Rosetti
Gasparoto Mancini, Maria Nadir
Hebling, Josimeri
de Souza Costa, Carlos Alberto
Marchi, Giselle Maria
Abstract: This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations. The amount (microg/mL) of hydrogen peroxide (H(2)O(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. Five groups (n = 10) were formed according to the CP concentration in the extracts: G1-DMEM (control); G2-0.0001% CP (0.025 microg/mL H(2)O(2)); G3-0.001% CP (0.43 microg/mL H(2)O(2)); G4-0.01% CP (2.21 microg/mL H(2)O(2)); and G5-0.1% CP (29.74 microg/mL H(2)O(2)). MDPC-23 cells were exposed to the bleaching gel extracts for 60 min and cell metabolism was evaluated by the MTT assay. Data were analyzed statistically by one-way ANOVA and Tukey's test (alpha = 0.05). Cell morphology was examined by scanning electron microscopy. The percentages of viable cells were as follows: G1, 100%; G2, 89.41%; G3, 82.4%; G4, 61.5%; and G5, 23.0%. G2 and G3 did not differ significantly (p > 0.05) from G1. The most severe cytotoxic effects were observed in G3 and G4. In conclusion, even at low concentrations, the CP gel extracts presented cytotoxic effects. This cytotoxicity was dose-dependent, and the 0.1% CP concentration caused the most intense cytopathic effects to the MDPC-23 cells.
Subject: Cell Line
Dental Enamel
Dose-response Relationship, Drug
Drug Combinations
Hydrogen Peroxide
Microscopy, Electron, Scanning
Tetrazolium Salts
Time Factors
Tooth Bleaching
Tooth Discoloration
Citation: Journal Of Biomedical Materials Research. Part B, Applied Biomaterials. v. 90, n. 2, p. 907-12, 2009-Aug.
Rights: fechado
Identifier DOI: 10.1002/jbm.b.31362
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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