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Type: Artigo de periódico
Title: Precipitation Of Porcine Insulin With Carbon Dioxide.
Author: Tashima, Alexandre Keiji
Ottens, Marcel
Van der Wielen, Luuk A M
Cintra, Dennys E
Pauli, José R
Filho, Pedro de Alcântara Pessôa
Miranda, Everson Alves
Abstract: Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical aspects of the precipitation of porcine insulin, a biomolecule of pharmaceutical interest, using CO2 as an acid-precipitating agent. The solubility of porcine insulin in NaHCO3 solutions in pressurized CO2 was determined as a function of temperature and pressure, with a minimum being observed close to the protein isoelectric point. A thermodynamic model was developed and successfully utilized to correlate the experimental data. Insulin was considered a polyelectrolyte in the model and its self-association reactions were also taken into account. The biological activity of insulin was maintained after precipitation with CO2, although some activity can be lost if foam is formed in the depressurization step.
Subject: Carbon Dioxide
Chemical Fractionation
Chemical Precipitation
Isoelectric Point
Citation: Biotechnology And Bioengineering. v. 103, n. 5, p. 909-19, 2009-Aug.
Rights: fechado
Identifier DOI: 10.1002/bit.22319
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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