Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/198254
Type: | Artigo de periódico |
Title: | Precipitation Of Porcine Insulin With Carbon Dioxide. |
Author: | Tashima, Alexandre Keiji Ottens, Marcel Van der Wielen, Luuk A M Cintra, Dennys E Pauli, José R Filho, Pedro de Alcântara Pessôa Miranda, Everson Alves |
Abstract: | Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical aspects of the precipitation of porcine insulin, a biomolecule of pharmaceutical interest, using CO2 as an acid-precipitating agent. The solubility of porcine insulin in NaHCO3 solutions in pressurized CO2 was determined as a function of temperature and pressure, with a minimum being observed close to the protein isoelectric point. A thermodynamic model was developed and successfully utilized to correlate the experimental data. Insulin was considered a polyelectrolyte in the model and its self-association reactions were also taken into account. The biological activity of insulin was maintained after precipitation with CO2, although some activity can be lost if foam is formed in the depressurization step. |
Subject: | Carbon Dioxide Carbonates Chemical Fractionation Chemical Precipitation Insulin Isoelectric Point |
Citation: | Biotechnology And Bioengineering. v. 103, n. 5, p. 909-19, 2009-Aug. |
Rights: | fechado |
Identifier DOI: | 10.1002/bit.22319 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/19347960 |
Date Issue: | 2009 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
---|---|---|---|
pmed_19347960.pdf | 215.78 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.