Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198250
Type: Artigo de periódico
Title: Studying Taxis In Real Time Using Optical Tweezers: Applications For Leishmania Amazonensis Parasites.
Author: Pozzo, L Y
Fontes, A
de Thomaz, A A
Santos, B S
Farias, P M A
Ayres, D C
Giorgio, S
Cesar, C L
Abstract: Beads trapped by an optical tweezers can be used as a force transducer for measuring forces of the same order of magnitude as typical forces induced by flagellar motion. We used an optical tweezers to study chemotaxis by observing the force response of a flagellated microorganism when placed in a gradient of attractive chemical substances. This report shows such observations for Leishmania amazonensis, responsible for leishmaniasis, a serious disease. We quantified the movement of this protozoan for different gradients of glucose. We were able to observe both the strength and the directionality of the force. The characterization of the chemotaxis of these parasites can help to understand the mechanics of infection and improve the treatments employed for this disease. This methodology can be used to quantitatively study the taxis of any kind of flagellated microorganisms under concentration gradients of different chemical substances, or even other types of variable gradients such as temperature and pressure.
Subject: Animals
Chemotactic Factors
Chemotaxis
Glucose
Leishmania Mexicana
Locomotion
Microscopy, Video
Optical Tweezers
Rights: fechado
Identifier DOI: 10.1016/j.micron.2009.02.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/19345110
Date Issue: -1-Uns- -1
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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