Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198246
Type: Artigo de periódico
Title: Mass Spectrometry Fingerprinting Of Media Used For In Vitro Production Of Bovine Embryos.
Author: Ferreira, Christina Ramires
Souza, Gustavo Henrique Martins Ferreira
Riccio, Maria Francesca
Catharino, Rodrigo Ramos
Pontes, José Henrique Fortes
Basso, Andréa Cristina
Júnior, José Carlos Ereno
Perecin, Felipe
Eberlin, Marcos Nogueira
Abstract: Using the bovine species as a biological model, direct infusion chip-based nano-electrospray ionization mass spectrometry (nano-ESI-MS) fingerprinting in the positive ion mode is used to obtain fast chemical profiles of media used for in vitro production of bovine embryos. Nano-ESI-MS fingerprinting is useful for characterization and routine quality control requiring no sample pre-separation, being able to differentiate four different media (IVM, IVF, SOF and HSOF) via principal component analysis (PCA). For media stored at +4 degrees C for up to 45 days, no significant (p>0.05) variation was observed in cleavage and blastocyst rate development, as well as in the nano-ESI-MS chemical profiles. For media exposed to a heat shock (60 degrees C for 3 h), no significant decrease (p>0.05) in embryo development rates was observed, but nano-ESI-MS profiles were quite distant from fresh control media in the PCA. For frozen media (-70 degrees C for 2 months), again no significant variation (p>0.05) in embryo development was noticed, but nano-ESI-MS profiles from all media were significantly affected. These results indicate that nano-ESI(+)-MS fingerprinting was able to characterize different media based on their specific chemical profile. The technique seems therefore applicable as a routine quality control assay, detecting, for example, compositional changes after temperature variations that may affect post-transfer embryo viability.
Subject: Animals
Cattle
Culture Media
Embryo Culture Techniques
Embryo, Mammalian
Spectrometry, Mass, Electrospray Ionization
Temperature
Time Factors
Citation: Rapid Communications In Mass Spectrometry : Rcm. v. 23, n. 9, p. 1313-20, 2009-May.
Rights: fechado
Identifier DOI: 10.1002/rcm.3995
Address: http://www.ncbi.nlm.nih.gov/pubmed/19338029
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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