Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198235
Type: Artigo de periódico
Title: Interferon Beta And Glatiramer Acetate Induce Proliferation Of Schwann Cells In Vitro.
Author: Graciele Zanon, Renata
Pierucci, Amauri
L R Oliveira, Alexandre
Abstract: Techniques as well as substances capable of stimulating cultured Schwann cell (SC) proliferation are needed for future therapeutical applications. In this work, the effects of interferon beta (IFNbeta) and glatiramer acetate (GA) on SC cultures were tested, with interest on the growth curve and potential proliferative effects. Primary cultures were prepared from the sciatic nerves of neonatal rats and seeded onto culture plates. Such cells were then subjected to treatment with different doses of IFN beta (100, 500 and 1000 IU/ml) and of GA (1.2, 2.5 and 5.0 microg/ml) for five consecutive days. S100beta and DAPI double labeling was used in order to confirm the purity of the cultures. Both treatment with IFN and GA resulted in an increased number of cultured SCs. However, only IFN beta induced a statistically significant proliferative outcome. Such results indicate that addition of IFN beta to the culture medium is efficient in order to improve SC proliferation in vitro.
Subject: Animals
Animals, Newborn
Cell Proliferation
Cells, Cultured
Dose-response Relationship, Drug
Immunologic Factors
Indoles
Interferon-beta
Mitosis
Peptides
Proliferating Cell Nuclear Antigen
Rats
Rats, Sprague-dawley
S100 Proteins
Schwann Cells
Sciatic Nerve
Time Factors
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/19325648
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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