Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198228
Type: Artigo de periódico
Title: Determination Of Short-chain Fatty Acids In Dietary Fiber Extracts Using Ion-exclusion Chromatography With Suppressed Conductivity Detection.
Author: Dias, Jailson C
Suzuki, Erika
de Albuquerque, Cibele L
Ferreira, Anderson L
Brito, Alba R M S
Kubota, Lauro T
Abstract: A new chromatographic method for the sequential determination of short-chain fatty acids is described. Acetic, propionic and butyric acids were determined in dietary fiber extracts using ion-exclusion chromatography equipped with inverse chemical suppression and conductivity detection. The best optimization of the chromatographic conditions were achieved when a 100 mm x 7.8 mm ion-exclusion column with a solution of 0.5 mmol L(-1) sulfuric acid as eluent in a flow rate of 0.6 mL min(-1) were employed. The organic acids were sequentially separated in less than 10 min with limits of detection ranging from 1 up to 7.5 micromol L(-1) and limits of quantification from 5 up to 25 micromol L(-1). The linearity of the analytical response was studied in the range of 0.005-10 mmol L(-1) for acetic acid and 0.025-10 mmol L(-1) for propionic and butyric acids with coefficients of determination (R(2)) ranging between 0.9985 and 0.9999. The method was tested and proved to be selective, precise, accurate, reproducible and highly sensitive. Finally, the method was applied in the analysis of biological samples.
Subject: Cations
Chromatography, Ion Exchange
Dietary Fiber
Electric Conductivity
Fatty Acids
Feces
Fermentation
Humans
Indicators And Reagents
Reproducibility Of Results
Citation: Journal Of Pharmaceutical And Biomedical Analysis. v. 49, n. 4, p. 1128-32, 2009-May.
Rights: fechado
Identifier DOI: 10.1016/j.jpba.2009.02.013
Address: http://www.ncbi.nlm.nih.gov/pubmed/19297112
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_19297112.pdf269.98 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.