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Type: Artigo de periódico
Title: Chronic Ethanol Intake Promotes Double Gluthatione S-transferase/transforming Growth Factor-alpha-positive Hepatocellular Lesions In Male Wistar Rats.
Author: Pires, Paulo Wagner
Furtado, Kelly Silva
Justullin, Luis Antonio
Rodrigues, Maria Aparecida Marchesan
Felisbino, Sergio Luis
Barbisan, Luis Fernando
Abstract: The chronic ethanol intake influence on the gluthatione S-transferase (GST-P) and transforming growth factor alpha (TGF-alpha) expression in remodeling/persistent preneoplastic lesions (PNLs) was evaluated in the resistant hepatocyte model. Male Wistar rats were allocated into five groups: G1, non-treated, fed water and chow ad libitum; G2, non-treated and pair-fed chow (restricted to match that of G3 group) and a maltodextrin (MD) solution in tap water (matched ethanol-derived calories); G3, fed 5% ethanol in drinking water and chow ad libitum; G4, diethylnitrosamine (DEN, 200 mg/kg, body weight) plus 200 parts per million of 2-acetylaminofluorene (2-AAF) for 3 weeks and pair-fed chow (restricted to match that of G5 group) and an MD solution in tap water (matched ethanol-derived calories); G5, DEN/2-AAF treatment, fed ethanol 5% and chow ad libitum. All animals were subjected to 70% partial hepatectomy at week 3 and sacrificed at weeks 12 or 22, respectively. Liver samples were collected for histological analysis or immunohistochemical expression of GST-P, TGF-alpha and proliferating cell nuclear antigen or zymography for matrix metalloproteinases-2 and -9. At the end of ethanol treatment, there was a significant increase in the percentage of liver area occupied by persistent GST-P-positive PNLs, the number of TGF-alpha-positive PNLs and the development of liver tumors in ethanol-fed and DEN/2-AAF-treated groups (G5 versus G4, P < 0.001). In addition, ethanol feeding led to a significant increase in cell proliferation mainly in remodeling and persistent PNLs with immunoreactivity for TGF-alpha at week 22 (P < 0.001). Gelatinase activities were not altered by ethanol treatment. The results demonstrated that ethanol enhances the selective growth of PNL with double expression of TGF-alpha and GST-P markers.
Subject: Animals
Glutathione Transferase
Liver Neoplasms, Experimental
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Precancerous Conditions
Rats, Wistar
Transforming Growth Factor Alpha
Citation: Cancer Science. v. 99, n. 2, p. 221-8, 2008-Feb.
Rights: aberto
Identifier DOI: 10.1111/j.1349-7006.2007.00677.x
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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