Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198169
Type: Artigo de periódico
Title: Strongyloides Venezuelensis: The Antigenic Identity Of Eight Strains For The Immunodiagnosis Of Human Strongyloidiasis.
Author: Machado, Eleuza Rodrigues
Faccioli, Lúcia Helena
Costa-Cruz, Julia Maria
Lourenço, Elaine Vicente
Roque-Barreira, Maria Cristina
Gonçalves-Pires, Maria do Rosário de Fátima
Ueta, Marlene Tiduko
Abstract: The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of L3 were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite.
Subject: Animals
Antibodies, Helminth
Antigens, Helminth
Enzyme-linked Immunosorbent Assay
Feces
Fluorescent Antibody Technique, Indirect
Humans
Immunoblotting
Immunoglobulin G
Larva
Rats
Rodentia
Sigmodontinae
Strongyloides
Strongyloidiasis
Rights: fechado
Identifier DOI: 10.1016/j.exppara.2007.12.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/18226812
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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