Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198148
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleProduction Of Calcium Oxalate Crystals By The Basidiomycete Moniliophthora Perniciosa, The Causal Agent Of Witches' Broom Disease Of Cacao.pt_BR
dc.contributor.authorRio, Maria Carolina S dopt_BR
dc.contributor.authorde Oliveira, Bruno Vpt_BR
dc.contributor.authorde Tomazella, Daniela P Tpt_BR
dc.contributor.authorSilva, José A Fracassi dapt_BR
dc.contributor.authorPereira, Gonçalo A Gpt_BR
unicamp.authorMaria Carolina S do Rio, Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, 13083-970, São Paulo, Brazil.pt_BR
unicamp.author.externalBruno V de Oliveira,pt
unicamp.author.externalDaniela P T de Tomazella,pt
unicamp.author.externalJosé A Fracassi da Silva,pt
unicamp.author.externalGonçalo A G Pereira,pt
dc.subjectAgaricalespt_BR
dc.subjectAmino Acid Sequencept_BR
dc.subjectAnimalspt_BR
dc.subjectCacaopt_BR
dc.subjectCalcium Oxalatept_BR
dc.subjectFungal Proteinspt_BR
dc.subjectHydrolasespt_BR
dc.subjectHyphaept_BR
dc.subjectMicroscopy, Electron, Scanningpt_BR
dc.subjectMicroscopy, Polarizationpt_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectPlant Diseasespt_BR
dc.subjectSequence Alignmentpt_BR
dc.subjectSpectrometry, X-ray Emissionpt_BR
dc.description.abstractOxalic acid has been shown as a virulence factor for some phytopathogenic fungi, removing calcium from pectin and favoring plant cell wall degradation. Recently, it was published that calcium oxalate accumulates in infected cacao tissues during the progression of Witches' Broom disease (WBD). In the present work we report that the hemibiotrophic basidiomycete Moniliophthora perniciosa, the causal agent of WBD, produces calcium oxalate crystals. These crystals were initially observed by polarized light microscopy of hyphae growing on a glass slide, apparently being secreted from the cells. The analysis was refined by Scanning electron microscopy and the compositon of the crystals was confirmed by energy-dispersive x-ray spectrometry. The production of oxalate by M. perniciosa was reinforced by the identification of a putative gene coding for oxaloacetate acetylhydrolase, which catalyzes the hydrolysis of oxaloacetate to oxalate and acetate. This gene was shown to be expressed in the biotrophic-like mycelia, which in planta occupy the intercellular middle-lamella space, a region filled with pectin. Taken together, our results suggest that oxalate production by M. perniciosa may play a role in the WBD pathogenesis mechanism.en
dc.relation.ispartofCurrent Microbiologypt_BR
dc.relation.ispartofabbreviationCurr. Microbiol.pt_BR
dc.date.issued2008-Aprpt_BR
dc.identifier.citationCurrent Microbiology. v. 56, n. 4, p. 363-70, 2008-Apr.pt_BR
dc.language.isoengpt_BR
dc.description.volume56pt_BR
dc.description.firstpage363-70pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0343-8651pt_BR
dc.identifier.doi10.1007/s00284-007-9091-7pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18172716pt_BR
dc.date.available2015-11-27T13:13:33Z-
dc.date.accessioned2015-11-27T13:13:33Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:13:33Z (GMT). No. of bitstreams: 1 pmed_18172716.pdf: 527524 bytes, checksum: 272a493ba0972e96498c2a3706c2f9ef (MD5) Previous issue date: 2008en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198148-
dc.identifier.idPubmed18172716pt_BR
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