Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198077
Type: Artigo de periódico
Title: Actin Cytoskeletal And Functional Studies Of The Proximal Convoluted Tubules After Preservation.
Author: Figueiredo, J F
Bertels, I M V
Gontijo, J A R
Abstract: Proximal tubule cells have specialized apical membranes with microvilli that provide an extensive surface area for unidirectional transport of solute from lumen to blood. The major structural solute component is F-actin, which interacts with transmembrane proteins, including ion transport molecules related to normal absorptive and secretory functions. Our study was to evaluate F-actin and fluid absorption (Jv) in proximal tubules after exposure to preservation solutions. In vitro microperfusion technique and immunohistochemistry analysis. 1. Absorptions were similar in 1- and 24-hour-preserved tubules, as well as in fresh tubules. The exception was tubules for 24 hours in Euro-Collins solution, which did not show absorption, suggesting that it was affected. 2. Fluorescence intensity of actin tubules preserved for 1 hour in both solutions showed similar values to each other and to the control group; tubules preserved for 24 hours in both solutions were similar to each other, although statistically different than the control group and those preserved for 1 hour in Belzer (UW) solution. There were differences among groups in the distribution of F-actin; Jv values were different for 24-hour preservation in each solution, whereas fluorescence intensity was similar in both 24-hour solutions. Thus, actin cytoskeleton was not responsible for it, because 24-hour preservation in UW showed Jv results comparable to the control group.
Subject: Absorption
Actin Cytoskeleton
Actins
Adenosine
Allopurinol
Animals
Cytoskeleton
Glutathione
Insulin
Kidney Tubules, Collecting
Kidney Tubules, Proximal
Male
Microscopy, Confocal
Microvilli
Models, Animal
Organ Preservation
Organ Preservation Solutions
Perfusion
Rabbits
Raffinose
Citation: Transplantation Proceedings. v. 40, n. 10, p. 3311-5, 2008-Dec.
Rights: fechado
Identifier DOI: 10.1016/j.transproceed.2008.06.060
Address: http://www.ncbi.nlm.nih.gov/pubmed/19100379
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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