Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198072
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampTabchoury, Cínthia Pereira Machadopt_BR
dc.contributor.authorunicampSousa, Maria Clara K.pt_BR
dc.contributor.authorunicampArthur, Rodrigo Alexpt_BR
dc.contributor.authorunicampMattos-Graner, Renata de Oliveirapt_BR
dc.contributor.authorunicampDel Bel Cury, Altair Antoninhapt_BR
dc.contributor.authorunicampCury, Jaime Aparecidopt_BR
dc.typeArtigopt_BR
dc.titleEvaluation of genotypic diversity of streptococcus mutans using distinct arbitrary primerspt_BR
dc.contributor.authorTabchoury, Cínthia Pereira Machadopt_BR
dc.contributor.authorSousa, Maria Clara K.pt_BR
dc.contributor.authorArthur, Rodrigo Alexpt_BR
dc.contributor.authorMattos-Graner, Renata Oliveirapt_BR
dc.contributor.authorDel Bel Cury, Altair Antoninhapt_BR
dc.contributor.authorCury, Jaime Aparecidopt_BR
dc.subjectGenótipopt_BR
dc.subjectSalivapt_BR
dc.subjectStreptococcus mutanspt_BR
dc.subject.otherlanguageGenotypept_BR
dc.subject.otherlanguageSalivapt_BR
dc.subject.otherlanguage Streptococcus mutanspt_BR
dc.description.abstractStreptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.en
dc.description.abstractStreptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteerspt
dc.relation.ispartofJournal of applied oral sciencept_BR
dc.relation.ispartofabbreviationJ. appl. oral sci.pt_BR
dc.publisher.cityBauru, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherUSP/Faculdade de Odontologia de Baurupt_BR
dc.date.issued2008pt_BR
dc.date.monthofcirculationNov./Dec.pt_BR
dc.identifier.citationJournal Of Applied Oral Science : Revista Fob. v. 16, n. 6, p. 403-7pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber6pt_BR
dc.description.firstpage403pt_BR
dc.description.lastpage407pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1678-7757pt_BR
dc.identifier.eissn1678-7765pt_BR
dc.identifier.doi10.1590/S1678-77572008000600009 pt_BR
dc.identifier.urlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572008000600009pt_BR
dc.description.sponsorshipFAPESP – FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1FAPESP – FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsordocumentnumber2006/02308-9pt_BR
dc.date.available2015-11-27T13:13:24Z-
dc.date.accessioned2015-11-27T13:13:24Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:13:24Z (GMT). No. of bitstreams: 1 pmed_19082399.pdf: 139671 bytes, checksum: 6f9ca9b5086084ceb5502c555dfcd27e (MD5) Previous issue date: null Bitstreams deleted on 2020-05-14T20:38:45Z: pmed_19082399.pdf,. Added 1 bitstream(s) on 2020-05-14T20:43:03Z : No. of bitstreams: 1 19082399.pdf: 479971 bytes, checksum: 9a1c9b1ccbe28edbd08078da4a8e7cd9 (MD5) Bitstreams deleted on 2020-06-05T14:32:19Z: 19082399.pdf,. Added 1 bitstream(s) on 2020-06-05T14:34:57Z : No. of bitstreams: 1 19082399.pdf: 479971 bytes, checksum: 9a1c9b1ccbe28edbd08078da4a8e7cd9 (MD5) Bitstreams deleted on 2020-06-30T18:47:34Z: 19082399.pdf,. Added 1 bitstream(s) on 2020-06-30T18:50:29Z : No. of bitstreams: 1 19082399.pdf: 216945 bytes, checksum: 5df7bfb9c13c583c63405b956fc46161 (MD5)en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198072-
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Diagnóstico Oralpt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordAP-PCRpt_BR
dc.subject.keywordArbitrary primerspt_BR
dc.identifier.source19082399-
dc.creator.orcid0000-0002-7660-5685pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-8309-8135pt_BR
dc.creator.orcid0000-0002-4329-0437pt_BR
dc.creator.orcid0000-0003-1046-5605pt_BR
dc.type.formArtigopt_BR
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