Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198072
Type: Artigo de periódico
Title: Evaluation Of Genotypic Diversity Of Streptococcus Mutans Using Distinct Arbitrary Primers.
Author: Tabchoury, Cínthia Pereira Machado
Sousa, Maria Clara K
Arthur, Rodrigo Alex
Mattos-Graner, Renata Oliveira
Del Bel Cury, Altair Antoninha
Cury, Jaime Aparecido
Abstract: Streptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.
Subject: Adult
Bacteriological Techniques
Dna Primers
Dna, Bacterial
Electrophoresis, Agar Gel
Ethidium
Fluorescent Dyes
Genetic Variation
Genotype
Humans
Polymerase Chain Reaction
Random Amplified Polymorphic Dna Technique
Saliva
Streptococcus Mutans
Virulence
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/19082399
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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