Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198067
Type: Artigo de periódico
Title: Simple And Sensitive Electroanalytical Method For The Determination Of Ascorbic Acid In Urine Samples Using Measurements In An Aqueous Cationic Micellar Medium.
Author: Dos Reis, Alaécio Pinheiro
Tarley, César Ricardo Teixeira
Mello, Lucilene Dornelles
Kubota, Lauro Tatsuo
Abstract: An electroanalytical study for the amperometric determination of ascorbic acid (AA) in a human urine sample at a bare glassy carbon electrode using the CPC (cetylpyridinium chloride) surfactant is described. Under the optimized conditions of E(pa), I(pa), CPC concentration and pH, the oxidation peak potential of the AA shifts towards a less positive potential, and the peak current increase in a significant way in the presence of the surfactant. Under the best conditions, the method provided a linear calibration curve to AA in a 0.1 mol L(-1) phosphate buffer solution (pH 7.0) in the concentration range from 5.0 x 10(-7) up to 4.3 x 10(-4) mol L(-1), with a detection limit of 2.0 x 10(-7) mol L(-1) and a high correlation coefficient (r = 0.9996). The recovery test presented values of 98 - 105%, suggesting a great potential of the proposed method for AA determination in complex samples, such as urine.
Subject: Ascorbic Acid
Carbon
Cetylpyridinium
Electrochemistry
Electrodes
Glass
Humans
Hydrogen-ion Concentration
Micelles
Oxidation-reduction
Sensitivity And Specificity
Surface-active Agents
Water
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/19075466
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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