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Type: Artigo de periódico
Title: A Genome Survey Of Moniliophthora Perniciosa Gives New Insights Into Witches' Broom Disease Of Cacao.
Author: Mondego, Jorge M C
Carazzolle, Marcelo F
Costa, Gustavo G L
Formighieri, Eduardo F
Parizzi, Lucas P
Rincones, Johana
Cotomacci, Carolina
Carraro, Dirce M
Cunha, Anderson F
Carrer, Helaine
Vidal, Ramon O
Estrela, Raíssa C
García, Odalys
Thomazella, Daniela P T
de Oliveira, Bruno V
Pires, Acássia Bl
Rio, Maria Carolina S
Araújo, Marcos Renato R
de Moraes, Marcos H
Castro, Luis A B
Gramacho, Karina P
Gonçalves, Marilda S
Neto, José P Moura
Neto, Aristóteles Góes
Barbosa, Luciana V
Guiltinan, Mark J
Bailey, Bryan A
Meinhardt, Lyndel W
Cascardo, Julio Cm
Pereira, Gonçalo A G
Abstract: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9x coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/cacao pathosystem.
Subject: Agaricales
Cluster Analysis
Dna, Fungal
Expressed Sequence Tags
Genes, Fungal
Genome, Fungal
Models, Genetic
Multigene Family
Plant Diseases
Sequence Alignment
Sequence Analysis, Dna
Rights: aberto
Identifier DOI: 10.1186/1471-2164-9-548
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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