Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198035
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePurification And Characterization Of A Trypsin Inhibitor From Plathymenia Foliolosa Seeds.pt_BR
dc.contributor.authorRamos, Vanessa da Silveirapt_BR
dc.contributor.authorSilva, Gilvânia de Souzapt_BR
dc.contributor.authorFreire, Maria das Graças Machadopt_BR
dc.contributor.authorMachado, Olga Lima Tavarespt_BR
dc.contributor.authorParra, José Roberto Postalipt_BR
dc.contributor.authorMacedo, Maria Lígia Rodriguespt_BR
unicamp.authorVanessa da Silveira Ramos, Departamento de Bioquímica/Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil.pt_BR
unicamp.author.externalGilvânia de Souza Silva,pt
unicamp.author.externalMaria das Graças Machado Freire,pt
unicamp.author.externalOlga Lima Tavares Machado,pt
unicamp.author.externalJosé Roberto Postali Parra,pt
unicamp.author.externalMaria Lígia Rodrigues Macedo,pt
dc.subjectAmino Acid Sequencept_BR
dc.subjectFabaceaept_BR
dc.subjectKineticspt_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectPlant Proteinspt_BR
dc.subjectSeedspt_BR
dc.subjectSequence Alignmentpt_BR
dc.subjectTrypsin Inhibitorspt_BR
dc.description.abstractA novel trypsin inhibitor (PFTI) was isolated from Plathymenia foliolosa (Benth.) seeds by gel filtration chromatography on a Sephadex G-100, DEAE-Sepharose, and trypsin-Sepharose columns. By SDSPAGE, PFTI yielded a single band with a M(r) of 19 kDa. PFTI inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants (K(i)) of 4 x 10(-8) and 1.4 x 10(-6) M, respectively. PFTI retained more than 50% of activity at up to 50 degrees C for 30 min, but there were 80 and 100% losses of activity at 60 and 70 degrees C, respectively. DTT affected the activity or stability of PFTI. The N-terminal amino acid sequence of PFTI showed a high degree of homology with various members of the Kunitz family of inhibitors. Anagasta kuehniella is found worldwide; this insect attacks stored grains and products of rice, oat, rye, corn, and wheat. The velvet bean caterpillar (Anticarsia gemmatalis) is considered the main defoliator pest of soybean in Brazil. Diatraea saccharalis, the sugar cane borer, is the major pest of sugar cane crops, and its caterpillar-feeding behavior, inside the stems, hampers control. PFTI showed significant inhibitory activity against trypsin-like proteases present in the larval midguts on A. kuehniella and D. saccharalis and could suppress the growth of larvae.en
dc.relation.ispartofJournal Of Agricultural And Food Chemistrypt_BR
dc.relation.ispartofabbreviationJ. Agric. Food Chem.pt_BR
dc.date.issued2008-Decpt_BR
dc.identifier.citationJournal Of Agricultural And Food Chemistry. v. 56, n. 23, p. 11348-55, 2008-Dec.pt_BR
dc.language.isoengpt_BR
dc.description.volume56pt_BR
dc.description.firstpage11348-55pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1520-5118pt_BR
dc.identifier.doi10.1021/jf802778bpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18991455pt_BR
dc.date.available2015-11-27T13:13:19Z-
dc.date.accessioned2015-11-27T13:13:19Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:13:19Z (GMT). No. of bitstreams: 1 pmed_18991455.pdf: 1677182 bytes, checksum: 581ce871b566c729ae373a685845a5ca (MD5) Previous issue date: 2008en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198035-
dc.identifier.idPubmed18991455pt_BR
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