Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197999
Type: Artigo de periódico
Title: Effects Of Sucrose On The Extracellular Matrix Of Plaque-like Biofilm Formed In Vivo, Studied By Proteomic Analysis.
Author: Paes Leme, A F
Bellato, C M
Bedi, G
Cury, A A Del Bel
Koo, H
Cury, J A
Abstract: Previous studies have shown that sucrose promotes changes in the composition of the extracellular matrix (ECM) of plaque-like biofilm (PLB), but its effect on protein expression has not been studied in vivo. Therefore, the protein compositions of ECM of PLB formed with and without sucrose exposure were analyzed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For this purpose, a crossover study was conducted during two phases of 14 days each, during which a volunteer wore a palatal appliance containing eight enamel blocks for PLB accumulation. In each phase, a 20% sucrose solution or distilled and deionized water (control) were extraorally dripped onto the blocks 8x/day. On the 14th day, the PLB were collected, the ECM proteins were extracted, separated by two-dimensional gel electrophoresis, digested by in-gel trypsin and MALDI-TOF MS analyzed. In the ECM of PLB formed under sucrose exposure, the following changes compared with the control PLB were observed: (1) the presence of upregulated proteins that may be involved in bacterial response to environmental changes induced by sucrose and (2) the absence of calcium-binding proteins that may partly explain the low inorganic concentration found in ECM of PLB formed under sucrose exposure. The findings showing that sucrose affected the ECM protein composition of PLB in vivo provide further insight into the unique cariogenic properties of this dietary carbohydrate.
Subject: Adult
Bacterial Proteins
Biofilms
Calcium-binding Proteins
Cariogenic Agents
Cross-over Studies
Dental Enamel
Dental Plaque
Electrophoresis, Gel, Two-dimensional
Extracellular Matrix
Humans
Hydrogen-ion Concentration
Protein Isoforms
Proteome
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Sucrose
Rights: fechado
Identifier DOI: 10.1159/000159607
Address: http://www.ncbi.nlm.nih.gov/pubmed/18832830
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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