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Type: Artigo de periódico
Title: A Decrease In Retinal Progenitor Cells Is Associated With Early Features Of Diabetic Retinopathy In A Model That Combines Diabetes And Hypertension.
Author: Lopes de Faria, Jacqueline Mendonça
Silva, Kamila Cristina
Boer, Patrícia Aline
Cavalcanti, Tiago Correa
Rosales, Mariana Aparecida Brunini
Ferrari, Ana Luiza
Lopes de Faria, José Butori
Abstract: Hyperglycemia and hypertension contribute to the development of diabetic retinopathy, and this may involve alterations in the normal retinal cell cycle. In this work, we examined the influence of diabetes and hypertension on retinal cell replication in vivo and the relationship between these changes and several early markers of diabetic retinopathy. Diabetes was induced with streptozotocin in 4- and 12-week-old spontaneously hypertensive rats (SHR) and their Wistar Kyoto (WKY) controls. The rats were killed 15 days later. Retinal cells stained with bromodeoxyuridine (BrdU) were seen in rats of both ages. In 12-week-old rats, the number of BrdU-positive retinal cells was higher in SHR than in WKY rats. After 15 days of diabetes mellitus, there was a marked reduction in cell replication only in diabetic SHR (p=0.007). The BrdU-positive cells expressed neural, glial, or vascular progenitor markers. There was greater expression of p27(Kip1) in the ganglion cell layer of both diabetic groups (p=0.05), whereas in the inner nuclear layer there was enhanced expression only in diabetic SHR (p=0.02). There was a marked increase in the retinal expression of fibronectin (p=0.04) and vascular endothelial growth factor (p=0.02) in diabetic SHR that was accompanied by blood-retinal barrier breakdown (p=0.01). Concomitant diabetes and hypertension attenuated the proliferation of retinal cells, and it is associated with an increase in p27(Kip1) expression, fibronectin accumulation, and blood-retinal barrier breakdown. The replicative retinal cells displayed characteristics of progenitor cells.
Subject: Aging
Blood-retinal Barrier
Blotting, Western
Capillary Permeability
Cell Count
Cyclin-dependent Kinase Inhibitor P27
Diabetic Retinopathy
Disease Models, Animal
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein
In Situ Nick-end Labeling
Rats, Inbred Shr
Rats, Inbred Wky
Stem Cells
Time Factors
Vascular Endothelial Growth Factor A
Rights: aberto
Identifier DOI: 
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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