Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197964
Type: Artigo de periódico
Title: Cytotoxicity Of Goniothalamin Enantiomers In Renal Cancer Cells: Involvement Of Nitric Oxide, Apoptosis And Autophagy.
Author: de Fátima, Angelo
Zambuzzi, Willian F
Modolo, Luzia V
Tarsitano, Christiane A B
Gadelha, Fernanda R
Hyslop, Stephen
de Carvalho, João Ernesto
Salgado, Ione
Ferreira, Carmen V
Pilli, Ronaldo A
Abstract: Goniothalamin is a styryllactone synthesized by plants of the genus Goniothalamus. The biological activities of this molecule, particularly its anti-protozoan, anti-fungal, and larvicidal properties, have received considerable attention. In this work, we investigated the action of the natural and synthetic enantiomers (R)-goniothalamin (1) and (S)-goniothalamin (ent-1) on cell viability, nitric oxide synthase (NOS) expression and activity, and the expression of selected proteins involved in apoptosis and autophagy in renal cancer cells. Both compounds were cytotoxic and decreased the mitochondrial function of renal cancer cells. However, the enantiomers differentially affected the expression/activity profiles of some signaling pathway mediators. Ent-1 (4 nM) was more potent than 1 (6.4 microM) in inhibiting constitutive NOS activity (54% and 59% inhibition, respectively), and both enantiomers decreased the protein expression of neuronal and endothelial NOS, as assessed by western blotting. Ent-1 and 1 caused down-regulation of Ras and TNFR1 and inhibition of protein serine/threonine phosphatase 2A (PP2A). Compound 1 markedly down-regulated Bcl2, an anti-apoptotic protein, and also induced PARP cleavage. Despite inducing an expressive down-regulation of Bax, ent-1 was also able to induce PARP cleavage. These results suggest that these compounds caused apoptosis in renal cancer cells. Interestingly, ent-1 enhanced the expression of LC3, a typical marker of autophagy. NFkappaB was down-regulated in 1-treated cells. Overall, these results indicate that the anti-proliferative activity of the two enantiomers on renal cancer cells involved distinct signaling pathways, apoptosis and autophagy as dominant responses towards 1 and ent-1, respectively.
Subject: Apoptosis
Autophagy
Cell Proliferation
Cell Survival
Dose-response Relationship, Drug
Down-regulation
Drug Screening Assays, Antitumor
Humans
Kidney Neoplasms
Mitochondria
Molecular Conformation
Nitric Oxide
Nitric Oxide Synthase
Oxygen Consumption
Protein Phosphatase 2
Proto-oncogene Proteins C-bcl-2
Pyrones
Receptors, Tumor Necrosis Factor, Type I
Stereoisomerism
Tumor Cells, Cultured
Ras Proteins
Rights: fechado
Identifier DOI: 10.1016/j.cbi.2008.08.003
Address: http://www.ncbi.nlm.nih.gov/pubmed/18771661
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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