Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197910
Type: Artigo de periódico
Title: Assessment Of The Diagnostic Potential Of Immmunocapture-pcr And Immuno-pcr For Citrus Variegated Chlorosis.
Author: Peroni, Luís Antonio
Reis, José Raimundo Ribeiro Dos
Coletta-Filho, Helvécio Della
de Souza, Alessandra Alves
Machado, Marcos Antonio
Stach-Machado, Dagmar Ruth
Abstract: Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
Subject: Antibodies, Bacterial
Citrus Sinensis
Culture Media
Enzyme-linked Immunosorbent Assay
Plant Diseases
Plant Leaves
Polymerase Chain Reaction
Sensitivity And Specificity
Xylella
Rights: fechado
Identifier DOI: 10.1016/j.mimet.2008.06.024
Address: http://www.ncbi.nlm.nih.gov/pubmed/18656503
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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