Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197874
Type: Artigo de periódico
Title: Characterization Of The C-terminal Half Of Human Juvenile Myoclonic Epilepsy Protein Efhc1: Dimer Formation Blocks Ca2+ And Mg2+ Binding To Its Functional Ef-hand.
Author: Murai, Marcelo J
Sassonia, Rogério C
Zamboni, André H
Conte, Fábio F
Martins-de-Souza, Daniel
Aparicio, Ricardo
de Oliveira, Marcelo G
Lopes-Cendes, Iscia
Abstract: Human EFHC1 is a member of the EF-hand superfamily of Ca(2+)-binding proteins with three DM10 domains of unclear function. Point mutations in the EFHC1 gene are related to juvenile myoclonic epilepsy, a fairly common idiopathic generalized epilepsy. Here, we report the first structural and thermodynamic analyses of the EFHC1C-terminus (residues 403-640; named EFHC1C), comprising the last DM10 domain and the EF-hand motif. Circular dichroism spectroscopy revealed that the secondary structure of EFHC1C is composed by 34% of alpha-helices and 17% of beta-strands. Size exclusion chromatography and mass spectrometry showed that under oxidizing condition EFHC1C dimerizes through the formation of disulfide bond. Tandem mass spectrometry (MS/MS) analysis of peptides generated by trypsin digestion suggests that the Cys575 is involved in intermolecular S-S bond. In addition, DTNB assay showed that each reduced EFHC1C molecule has one accessible free thiol. Isothermal titration calorimetry (ITC) showed that while the interaction between Ca(2+) and EFHC1C is enthalpically driven (DeltaH=-58.6 to -67 kJ/mol and TDeltaS=-22.5 to -31 kJ/mol) the interaction between Mg(2+) and EFHC1C involves an entropic gain, and is approximately 5 times less enthalpically favorable (DeltaH=-11.7 to -14 kJ/mol and TDeltaS=21.9 to 19 kJ/mol) than for Ca(2+) binding. It was also found that under reducing condition Ca(2+) or Mg(2+) ions bind to EFHC1C in a 1/1 molar ratio, while under oxidizing condition this ratio is reduced, showing that EFHC1C dimerization blocks Ca(2+) and Mg(2+) binding.
Subject: Amino Acid Sequence
Base Sequence
Binding Sites
Blotting, Western
Calcium
Calcium-binding Proteins
Chromatography, Gel
Dna Primers
Dimerization
Humans
Magnesium
Mass Spectrometry
Molecular Sequence Data
Myoclonic Epilepsy, Juvenile
Protein Binding
Protein Structure, Secondary
Rights: fechado
Identifier DOI: 10.1016/j.abb.2008.06.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/18593566
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_18593566.pdf708.83 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.