Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197828
Type: Artigo de periódico
Title: In Vitro Evaluation Of The Effect Of Nicotine, Cotinine, And Caffeine On Oral Microorganisms.
Author: Cogo, Karina
Montan, Michelle Franz
Bergamaschi, Cristiane de Cássia
D Andrade, Eduardo
Rosalen, Pedro Luiz
Groppo, Francisco Carlos
Abstract: The aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. It also evaluated the ability of these bacteria to metabolize those substances. Single-species biofilms of Streptococcus gordonii, Porphyromonas gingivalis, or Fusobacterium nucleatum and dual-species biofilms of S. gordonii -- F. nucleatum and F. nucleatum -- P. gingivalis were grown on hydroxyapatite discs. Seven species were studied as planktonic cells, including Streptococcus oralis, Streptococcus mitis, Propionibacterium acnes, Actinomyces naeslundii, and the species mentioned above. The viability of planktonic cells and biofilms was analyzed by susceptibility tests and time-kill assays, respectively, against different concentrations of nicotine, cotinine, and caffeine. High-performance liquid chromatography was performed to quantify nicotine, cotinine, and caffeine concentrations in the culture media after the assays. Susceptibility tests and viability assays showed that nicotine, cotinine, and caffeine cannot reduce or stimulate bacterial growth. High-performance liquid chromatography results showed that nicotine, cotinine, and caffeine concentrations were not altered after bacteria exposure. These findings indicate that nicotine, cotinine, and caffeine, in the concentrations used, cannot affect significantly the growth of these oral bacterial strains. Moreover, these species do not seem to metabolize these substances.
Subject: Bacteria
Biofilms
Caffeine
Cotinine
Dose-response Relationship, Drug
Humans
Microbial Viability
Mouth
Nicotine
Periodontal Diseases
Rights: fechado
Identifier DOI: 10.1139/w08-032
Address: http://www.ncbi.nlm.nih.gov/pubmed/18535638
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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