Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197772
Type: Artigo de periódico
Title: Electrocatalytic Activity Of 4-nitrophthalonitrile-modified Electrode For The L-glutathione Detection.
Author: Lima, Phabyanno R
Santos, Wilney J R
Oliveira, Adriano B
Goulart, Marília O F
Kubota, Lauro T
Abstract: The present work describes the substantial electrocatalytic activity of (NC)2C6H3-NHOH/(NC)2C6H3-NO redox couple-modified electrode toward the low voltage detection of l-glutathione (GSH), in neutral medium, at an applied potential of 0.4V versus Ag/AgCl. After optimizing the operational conditions, the sensor provided a linear response range for GSH from 8.0 up to 83.0 micromol L(-1) with sensitivity, detection and quantification limits of 54nA L micromol(-1), 2.7 micromol L(-1) and 8.0 micromol L(-1), respectively. The proposed sensor presented higher sensitivity when compared to other modified electrodes described in the literature and showed a stable response for at least 100 successive determinations. The repeatability of the measurements with the same sensor and different sensors, evaluated in terms of relative standard deviation, were 4.1 and 5.0%, respectively, for n=10. The developed sensor was applied for GSH determination in yeast extract and the results were statistically the same with those obtained by the comparative method described in the literature at a confidence level of 95%.
Subject: Biosensing Techniques
Buffers
Catalysis
Electrochemistry
Electrodes
Feasibility Studies
Glutathione
Hydrogen-ion Concentration
Nitriles
Oxidation-reduction
Reproducibility Of Results
Saccharomyces Cerevisiae
Sensitivity And Specificity
Rights: fechado
Identifier DOI: 10.1016/j.jpba.2008.03.006
Address: http://www.ncbi.nlm.nih.gov/pubmed/18434067
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_18434067.pdf323.55 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.