Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197764
Type: Artigo de periódico
Title: Association Of Tgf-beta1, Cd14, Il-4, Il-4r And Adam33 Gene Polymorphisms With Asthma Severity In Children And Adolescents.
Author: de Faria, Isabel C J
de Faria, Elisangela J
Toro, Adyléia A D C
Ribeiro, José Dirceu
Bertuzzo, Carmen Silvia
Abstract: To verify the association of transforming growth factor-beta1(TGF-beta1) (C-509T and T869C), CD14 (C-159T), IL-4 (C-590T), IL-4R (ILe50Val) and ADAM33 (S_2) gene polymorphisms with asthma severity in a sample of patients with mild, moderate and severe persistent atopic asthma. A clinical, laboratory, prospective study was performed in patients with persistent atopic asthma, compared to a control group at Hospital Universitário da Universidade Estadual de Campinas between 2006 and 2007. Analysis of the TGF-beta1 T869C gene polymorphism was performed using the technique polymerase chain reaction (PCR) + amplification refractory mutation system (ARMS). TGF-beta1 C-509T, CD14 C-159T, IL-4 C-590T, IL-4Ra ILe50Val, and ADAM33 S2 gene polymorphisms were detected by PCR and restriction enzyme. This study included 88 patients with persistent atopic asthma (27 mild, 23 moderate and 38 severe) and 202 healthy blood donors. As to T869C polymorphism (TGF-beta1), there was an association between the CC genotype and patients with severe asthma. There was no association in polymorphisms C-509T (TGF-beta1), C-590T (IL-4) and S_2 (ADAM33). When distribution of C-159T polymorphism genotype frequency (CD14) in severe asthma was compared with the control group, there was a significant result with the TT genotype. There was significant association of the Val/Val genotype (IL-4R) with mild asthma. Our results indicate that T869C (TGF-beta1), C-159T (CD14) and Val/Val (IL-4R) polymorphisms might be involved in modulation of asthma severity.
Subject: Adam Proteins
Adolescent
Antigens, Cd14
Asthma
Case-control Studies
Child
Cytokines
Female
Genetic Markers
Genotype
Humans
Interleukin-4
Male
Phenotype
Polymerase Chain Reaction
Polymorphism, Genetic
Prospective Studies
Receptors, Immunologic
Receptors, Interleukin-4
Severity Of Illness Index
Transforming Growth Factor Beta1
Rights: aberto
Identifier DOI: doi:10.2223/JPED.1783
Address: http://www.ncbi.nlm.nih.gov/pubmed/18425216
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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