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Type: Artigo de periódico
Title: Monoglycerides And Diglycerides Synthesis In A Solvent-free System By Lipase-catalyzed Glycerolysis.
Author: Fregolente, Patricia Bogalhos Lucente
Fregolente, Leonardo Vasconcelos
Pinto, Gláucia Maria F
Batistella, Benedito César
Wolf-Maciel, Maria Regina
Filho, Rubens Maciel
Abstract: Five lipases were screened (Thermomyces lanuginosus free and immobilized forms, Candida antarctica B, Candida rugosa, Aspergillus niger, and Rhizomucor miehei) to study their ability to produce monoglycerides (MG) and diglycerides (DG) through enzymatic glycerolysis of soybean oil. Lipase from C. antarctica was further studied to verify the enzyme load (wt% of oil mass), the molar ratio glycerol/oil, and the water content (wt% of glycerol) on the glycerolysis reaction. The best DG and MG productions were in the range 45-48% and 28-30% (w/w, based on the total oil), respectively. Using immobilized lipases, the amount of free fatty acids (FFA) produced was about 5%. However, the amount of FFA produced when using free lipases, with 3.5% extra water in the system, is equivalent to the MG yield, about 23%. The extra water content provides a competition between hydrolysis and glycerolysis reactions, increasing the FFA production.
Subject: Diglycerides
Enzyme Activation
Enzyme Stability
Enzymes, Immobilized
Fatty Acids, Nonesterified
Fungal Proteins
Soybean Oil
Substrate Specificity
Citation: Applied Biochemistry And Biotechnology. v. 146, n. 1-3, p. 165-72, 2008-Mar.
Rights: fechado
Identifier DOI: 10.1007/s12010-008-8133-3
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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