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http://repositorio.unicamp.br/jspui/handle/REPOSIP/197762
Type: | Artigo de periódico |
Title: | Monoglycerides And Diglycerides Synthesis In A Solvent-free System By Lipase-catalyzed Glycerolysis. |
Author: | Fregolente, Patricia Bogalhos Lucente Fregolente, Leonardo Vasconcelos Pinto, Gláucia Maria F Batistella, Benedito César Wolf-Maciel, Maria Regina Filho, Rubens Maciel |
Abstract: | Five lipases were screened (Thermomyces lanuginosus free and immobilized forms, Candida antarctica B, Candida rugosa, Aspergillus niger, and Rhizomucor miehei) to study their ability to produce monoglycerides (MG) and diglycerides (DG) through enzymatic glycerolysis of soybean oil. Lipase from C. antarctica was further studied to verify the enzyme load (wt% of oil mass), the molar ratio glycerol/oil, and the water content (wt% of glycerol) on the glycerolysis reaction. The best DG and MG productions were in the range 45-48% and 28-30% (w/w, based on the total oil), respectively. Using immobilized lipases, the amount of free fatty acids (FFA) produced was about 5%. However, the amount of FFA produced when using free lipases, with 3.5% extra water in the system, is equivalent to the MG yield, about 23%. The extra water content provides a competition between hydrolysis and glycerolysis reactions, increasing the FFA production. |
Subject: | Diglycerides Enzyme Activation Enzyme Stability Enzymes, Immobilized Fatty Acids, Nonesterified Fungal Proteins Glycerol Lipase Monoglycerides Solvents Soybean Oil Substrate Specificity |
Citation: | Applied Biochemistry And Biotechnology. v. 146, n. 1-3, p. 165-72, 2008-Mar. |
Rights: | fechado |
Identifier DOI: | 10.1007/s12010-008-8133-3 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/18421596 |
Date Issue: | 2008 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
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pmed_18421596.pdf | 119.31 kB | Adobe PDF | View/Open |
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