Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197758
Type: Artigo de periódico
Title: Hematopoietic Response Of Rats Exposed To The Impact Of An Acute Psychophysiological Stressor On Responsiveness To An In Vivo Challenge With Listeria Monocytogenes: Modulation By Chlorella Vulgaris Prophylactic Treatment.
Author: Queiroz, Julia de Souza
Torello, Cristiane Okuda
Palermo-Neto, João
Valadares, Marize C
Queiroz, Mary L S
Abstract: In this study, we investigated the hematopoietic response of rats pretreated with CV and exposed to the impact of acute escapable, inescapable or psychogenical stress on responsiveness to an in vivo challenge with Listeria monocytogenes. No consistent changes were observed after exposure to escapable footshock. Conversely, the impact of uncontrollable stress (inescapable and psychogenical) was manifested by an early onset and increased severity and duration of myelossuppression produced by the infection. Small size CFU-GM colonies and increased numbers of clusters were observed, concurrently to a greater expansion in the more mature population of bone marrow granulocytes. No differences were observed between the responses of both uncontrollable stress regimens. CV prevented the myelossuppression caused by stress/infection due to increased numbers of CFU-GM in the bone marrow. Colonies of cells tightly packed, with a very condensed nucleus; in association with a greater expansion in the more immature population of bone marrow granulocytes were observed. Investigation of the production of colony-stimulating factors revealed increased colony-stimulating activity (CSA) in the serum of normal and infected/stressed rats treated with the algae. CV treatment restored/enhanced the changes produced by stress/infection in total and differential bone marrow and peripheral cells counts. Further studies demonstrated that INF-gamma is significantly reduced, whereas IL-10 is significantly increased after exposure to uncontrollable stress. Treatment with CV significantly increased INF-gamma levels and diminished the levels of IL-10. Uncontrollable stress reduced the protection afforded by CV to a lethal dose of L. monocytogenes, with survival rates being reduced from (50%) in infected rats to 20% in infected/stressed rats. All together, our results suggest Chlorella treatment as an effective tool for the prophylaxis of post-stress myelossupression, including the detrimental effect of stress on the course and outcome of infections.
Subject: Acute Disease
Animals
Behavior, Animal
Bone Marrow Cells
Chlorella Vulgaris
Colony-stimulating Factors
Electroshock
Escape Reaction
Granulocytes
Hematopoietic System
Interleukin-10
Interleukin-3
Listeria Monocytogenes
Listeriosis
Male
Rats
Rats, Wistar
Stress, Psychological
Survival Analysis
Time Factors
Rights: fechado
Identifier DOI: 10.1016/j.bbi.2008.03.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/18420376
Date Issue: 2008
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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