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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDifferent Urea Stoichiometries Between The Dissociation And Denaturation Of Tobacco Mosaic Virus As Probed By Hydrostatic Pressure.pt_BR
dc.contributor.authorSantos, Jose L Rpt_BR
dc.contributor.authorAparicio, Ricardopt_BR
dc.contributor.authorJoekes, Inéspt_BR
dc.contributor.authorSilva, Jerson Lpt_BR
dc.contributor.authorBispo, Jose A Cpt_BR
dc.contributor.authorBonafe, Carlos F Spt_BR
unicamp.authorJose L R Santos, Laboratório de Termodinâmica de Proteínas, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, Brazil.pt_BR
unicamp.author.externalRicardo Aparicio,pt
unicamp.author.externalInés Joekes,pt
unicamp.author.externalJerson L Silva,pt
unicamp.author.externalJose A C Bispo,pt
unicamp.author.externalCarlos F S Bonafe,pt
dc.subjectChromatography, Gelpt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectHydrostatic Pressurept_BR
dc.subjectMicroscopy, Electron, Transmissionpt_BR
dc.subjectMolecular Conformationpt_BR
dc.subjectTobacco Mosaic Viruspt_BR
dc.subjectTryptophanpt_BR
dc.subjectUreapt_BR
dc.subjectWaterpt_BR
dc.description.abstractViruses are very efficient self-assembly structures, but little is understood about the thermodynamics governing their directed assembly. At higher levels of pressure or when pressure is combined with urea, denaturation occurs. For a better understanding of such processes, we investigated the apparent thermodynamic parameters of dissociation and denaturation by assuming a steady-state condition. These processes can be measured considering the decrease of light scattering of a viral solution due to the dissociation process, and the red shift of the fluorescence emission spectra, that occurs with the denaturation process. We determined the apparent urea stoichiometry considering the equilibrium reaction of TMV dissociation and subunit denaturation, which furnished, respectively, 1.53 and 11.1 mol of urea/mol of TMV subunit. The denaturation and dissociation conditions were arrived in a near reversible pathway, allowing the determination of thermodynamic parameters. Gel filtration HPLC, electron microscopy and circular dichroism confirmed the dissociation and denaturation processes. Based on spectroscopic results from earlier papers, the calculation of the apparent urea stoichiometry of dissociation and denaturation of several other viruses resulted in similar values, suggesting a similar virus-urea interaction among these systems.en
dc.relation.ispartofBiophysical Chemistrypt_BR
dc.relation.ispartofabbreviationBiophys. Chem.pt_BR
dc.date.issued2008-Maypt_BR
dc.identifier.citationBiophysical Chemistry. v. 134, n. 3, p. 214-24, 2008-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume134pt_BR
dc.description.firstpage214-24pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0301-4622pt_BR
dc.identifier.doi10.1016/j.bpc.2008.02.010pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18367310pt_BR
dc.date.available2015-11-27T13:12:37Z-
dc.date.accessioned2015-11-27T13:12:37Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:12:37Z (GMT). No. of bitstreams: 1 pmed_18367310.pdf: 1817698 bytes, checksum: 6cefbd7c35a38bd32db3e1d69bff84c6 (MD5) Previous issue date: 2008en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197721-
dc.identifier.idPubmed18367310pt_BR
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