Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197712
Type: Artigo de periódico
Title: High Susceptibility Of Activated Lymphocytes To Oxidative Stress-induced Cell Death.
Author: Degasperi, Giovanna R
Castilho, Roger F
Vercesi, Anibal E
Abstract: The present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in activated lymphocytes increases their susceptibility to oxidative stress-induced cell death in a mechanism involving the participation of mitochondrial permeability transition.
Subject: Animals
Apoptosis
Calcium
Carcinoma 256, Walker
Chelating Agents
Deferoxamine
Egtazic Acid
Flow Cytometry
Lymphocyte Activation
Lymphocytes
Male
Membrane Potentials
Mitochondria
Nifedipine
Oxidation-reduction
Oxidative Stress
Rats
Rats, Wistar
Siderophores
Spleen
Time Factors
Tert-butylhydroperoxide
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/18345382
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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