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Type: Artigo de periódico
Title: Gemella Morbillorum In Primary And Secondary/persistent Endodontic Infections.
Author: Gomes, Brenda P F A
Montagner, Francisco
Jacinto, Rogério Castilho
Pinheiro, Ericka Tavares
Zaia, Alexandre A
Ferraz, Caio Cezar Randi
Souza-Filho, Francisco J
Abstract: To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections. Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers. Culture and polymerase chain reaction (PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 (38%) of root canal samples from primary and in 4 of 50 (8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 (82%) and 36 of 50 (72%) of the, respectively, primary and secondary/persistent root canal infections studied. Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.
Subject: Chi-square Distribution
Dna, Bacterial
Dental Pulp Cavity
Periapical Abscess
Periapical Periodontitis
Root Canal Irrigants
Root Canal Therapy
Citation: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, And Endodontics. v. 105, n. 4, p. 519-25, 2008-Apr.
Rights: fechado
Identifier DOI: 10.1016/j.tripleo.2007.10.005
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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