Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197652
Type: Artigo de periódico
Title: Combination Of Angiotensin-converting Enzyme And Methylenetetrahydrofolate Reductase Gene Polymorphisms As Determinant Risk Factors For Chronic Allograft Dysfunction.
Author: de Alvarenga, M P S
Pavarino-Bertelli, E C
Abbud-Filho, M
Ferreira-Baptista, M A S
Haddad, R
Eberlin, M N
Goloni-Bertollo, E M
Abstract: The aim of this study was to investigate the frequency of gene angiotensin-converting enzyme insertion/deletion (ACE I/D) and methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) variants, as well as to evaluate the plasma homocysteine concentrations in 217 patients who underwent renal transplantation at least 12 months prior to define risk factors for chronic allograft dysfunction. The presence of the polymorphism ACE deletion was assessed by polymerase chain reaction (PCR) analysis. MTHFR polymorphisms were determined by PCR and restriction fragment length polymorphism (RFPL) techniques. The restriction enzymes were Hinf I and Mbo II for MTHFR variants C677T and A1298C, respectively. Plasma homocysteine concentrations were measured by liquid chromatography-tandem mass spectrometry (LS-MS/MS). Hyperhomocysteinemias were more common in patients with chronic allograft dysfunction (P = .004). No statistically significant differences were observed between the allelic and genotypic distributions of MTHFR and ACE polymorphisms. An effective risk factor was found when the polymorphisms of the ACE and MTHFR genes and hyperhomocysteinemia were associated (odds ratio 2.51; 95% confidence interval 1.19-5.28). In conclusion, our study identified that the presence of hyperhomocysteinemia in combination with unfavorable genotypes contributes to an increased risk for development of chronic allograft dysfunction.
Subject: Adult
Chronic Disease
Creatinine
Cross-sectional Studies
Female
Gene Deletion
Genotype
Humans
Hyperhomocysteinemia
Kidney Transplantation
Male
Methylenetetrahydrofolate Reductase (nadph2)
Middle Aged
Peptidyl-dipeptidase A
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Postoperative Complications
Risk Factors
Transplantation, Homologous
Rights: fechado
Identifier DOI: 10.1016/j.transproceed.2006.10.224
Address: http://www.ncbi.nlm.nih.gov/pubmed/17275478
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_17275478.pdf67.37 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.